Fluidigm Juno - How to Use the Juno Touchscreen

Genotyping with Juno Getting Started Guide
20
Chapter 3: Genotyping with the Juno
96.96 Genotyping IFC Using SNP Type
Assays
Prepare Assay and Sample Mixes
Prepare the 200 nM Primer Pool for Preamplification
1 In a new 1.5-mL microcentrifuge tube, combine 2 μL of 100 μM SNP Type assays
specific target amplification primers (100 μM STA) up to a total of 96 assays. The total
volume is Y in Table 4.
2 In the same microcentrifuge tube, combine 2 μL of 100 μM SNP Type assays locus-
specific primers (100 μM LSP) up to a total of 96 assays. The total volume is Z in
Table 4.
3 Add Dilution Reagent to the SNP Type assays:
Table 4. Pool SNP Type assays
NOTE
• Volume can be adjusted proportionally based on the number of
samples to be amplified.
• You can store the pooled SNP Type STA assays at –20 °C for 1 year or ≤10
freeze-thaw cycles, whichever is shorter.
Component Volume (μL) Final Concentration
(nM*)
* The final concentration of each primer in the preamplification reaction is 50 nM.
SNP Type assays specific target amplification
primers (100 μM STA)
Y (up to 96 assays) 200.0
SNP Type assays locus-specific primers
(100 μM LSP)
Z (up to 96 assays) 200.0
Dilution Reagent 1,000 – (Y + Z)—
Total 1,000.0 —