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Fluidigm Juno - Chapter 1: Product Information; Required Kit Contents; Taqman Assay Kit

Fluidigm Juno
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Genotyping with Juno Getting Started Guide
8
Chapter 1: Product Information
Required Kit Contents
The kits include the reagents required for preparing 10 IFCs to use on the Juno
system. For suggested kits, see “Suggested Kits” on page 28.
IMPORTANT
Do not pipet reagents from the TaqMan and SNPType assay kits into the same IFC.
Use a different IFC for each kit. Do not mix reagents from different kits.
Unless otherwise specified, thaw reagents at room temperature, then use them at
room temperature. Store reagents at their specified storage temperatures. Vortex
reagents for 20 seconds, then centrifuge reagents for 2 seconds before use.
TaqMan Assay Kit
Figure 1. Juno™ Genotyping Kit for 10 IFCs (PN 100-8362).
Box Component Cap Color Quantity Volume
per Tube (mL)
Storage
Juno Genotyping Kit
for 10 IFCs
(PN 100-8362)
Juno GT Preamp
Master Mix
Light
purple
1 tube 1.35
–20 ºC
Dilution Reagent Natural 2 tubes 1.7
Probe GT Master Mix Gold 2 tubes 1.6
Juno GT Flux Fluid Purple 1 tube 0.9
Juno 96.96
Genotyping IFC—
10 IFCs
10 IFCs
Room
temperature
Juno 96.96 GT
Control Line Fluid
2 boxes;
20 syringes/
box
Probe GT Master Mix
Juno GT Flux Fluid
Juno GT Preamp Master Mix
Dilution Reagent

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