Genotyping with Juno Getting Started Guide
3
Contents
About This Guide 4
Purpose 4
How to Use This Guide 5
Safety Alert Conventions 5
Safety Alerts for Chemicals 5
Safety Alerts for Instruments 5
Safety Data Sheets 6
Getting Started 7
Workflow 7
Best Practices 7
Related Documentation 7
Chapter 1: Product Information 8
Required Kit Contents 8
TaqMan Assay Kit 8
SNP Type Assay Kit 9
Required Reagents 10
TaqMan Assays 10
SNP Type Assays 10
Suggested Reagents 10
Required Consumables 11
Required Equipment 11
Suggested Equipment 11
Required Software 12
IFC Type and Related Scripts 12
Chapter 2: Genotyping with the
Juno 96.96 Genotyping IFC Using
TaqMan Assays 13
Prepare Assay and Sample Mixes 13
Prepare the Primer Pool for Preamplification 13
Prepare 2X TaqMan Assays for Genotyping 14
Prepare the Assay Mix 14
Obtain the Minimum Required Genomic DNA 14
Prepare the Sample Mix 15
Load and Run the IFC on Juno 16
Perform Genotyping Analysis on the Samples 19
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Chapter 3: Genotyping with the Juno
96.96 Genotyping IFC Using SNP
Type Assays 20
Prepare Assay and Sample Mixes 20
Prepare the 200 nM Primer Pool for
Preamplification 20
Prepare 2X SNP Type Assays 21
Prepare the Assay Mix 22
Obtain the Minimum Required Genomic DNA 22
Prepare the Sample Mix 23
Load and Run the IFC on Juno 24
Perform Genotyping Analysis on the Samples 27
27
Appendix A: Suggested Kits 28
TaqMan Assay Kit 28
SNP Type Assay Kit 28
Suggested Reagents to Use with TaqMan
Assay and SNP Type Assay Kits 29
Appendix B: Safety 30
General Safety 30
Instrument Safety 30
Electrical Safety 31
Chemical Safety 31
Disposal of Products 31
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