Genotyping with Juno Getting Started Guide
Chapter 3: Genotyping with the Juno 96.96 Genotyping IFC Using SNP Type Assays
Prepare Assay and Sample Mixes
21
Prepare 2X SNP Type Assays
Prepare 50X Primer Mix for Each Single Assay Inlet
In a DNA-free hood, in a new 96-well plate, combine the following reagents for each
assay:
Prepare 2X SNP Type Assays from the 50X Primer Mix for
Genotyping
In a DNA-free hood, in a new 96-well plate, combine the following reagents for each
assay:
NOTE You can store the 2X SNP Type assays at –20 °C for up to one week.
Component Volume per 40 μL
Stock (μL)
Final Concentration
(μM)
SNP Type assays allele-specific primers
pooled ASP1 and ASP2 Primers (100 μM
ASP1/100 μM ASP2)
3.0 7.5
SNP Type assays locus-specific primers
(100 μM LSP)
8.0 20.0
Dilution Reagent 29.0 —
Total 40.0*
* A 40.0-μL volume is sufficient for 40, 2X SNP Type assays.
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Component Volume per 25 μL
Stock (μL)
Final
Concentration
50X Primer Mix*
* See “Prepare 50X Primer Mix for Each Single Assay Inlet”.
1.0 2X
Dilution Reagent 24.0 —
Total 25.0†
† A 25.0-μL volume is sufficient for 10 IFC runs.
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