What to do if the Fluidigm CyTOF XT LED status panel is not lit?
If the LED status panel isn't lit, it indicates a power issue. First, ensure the system power cord is fully inserted into the power source. If the problem persists, contact your facilities department to check for power interruptions. Also, check the system power circuit breakers (black) on the right side of the instrument and switch it on if it's off.
Why does Fluidigm CyTOF XT plasma not ignite or stay lit?
Several factors can prevent the plasma from igniting or staying lit. Start by checking the RFG power circuit breaker (red) on the right side of the instrument and switch it on. Also, verify with the vendor that argon (with purity ?99.996%) is being supplied to the instrument. Ensure that the argon supply is steady and proper argon pressure is maintained by checking the argon regulator on the tank (approximately 80 psi). If using a liquid argon tank, ensure the pressure build valve is open. Finally, check the exhaust flow reading in the CyTOF Software: Instrument Configuration > Plasma and RFG > Gases> Exhaust Flow Rate (cfm) = 80 ± 10%. Ensure proper and consistent exhaust by checking with your laboratory facilities manager.
What to do if Fluidigm Laboratory Equipment loses communication with the computer?
If communication is lost between the instrument and the computer, first, unplug the thunderbolt connection from the back of the computer and plug it back in. If that doesn't work, power off both the instrument and the computer for at least 10 seconds before powering them back on.
What to do if the Fluidigm Laboratory Equipment has failures in automated tuning?
If automated tuning fails, it could be due to insufficient signal during tuning. Check that EQ™ Six Element Calibration Beads (EQ6) beads are loaded in the correct location in the carousel. Add or replace the beads if needed, and vigorously shake the bottle of EQ6 beads before transferring them to the tube.
What to do if Fluidigm CyTOF XT Laboratory Equipment has no/poor vacuum?
If there is no or poor vacuum, check the right side of the instrument to ensure that the Vacuum button is illuminated. This ensures that the vacuum pumps are turned on.
Explains Fluidigm's conventions for presenting safety information requiring user attention.
Details chemical hazard indicators, pictograms, and signal words based on GHS standards.
Describes instrument hazard indicators, pictograms, and signal words for severity levels.
Instructs users on obtaining and understanding Safety Data Sheets (SDS) for chemicals.
Details the physical and environmental requirements for installing the CyTOF XT instrument.
Provides technical specifications for the CyTOF XT instrument's performance and capabilities.
Lists the hardware specifications required for the workstation controlling the CyTOF XT.
Outlines the expected file sizes for different data types generated by the instrument.
Illustrates the necessary connections between the instrument and the computer.
Lists the standard equipment and consumables included with the CyTOF XT system.
Details the primary equipment components that come standard with the CyTOF XT.
Lists the standard consumables (parts) for the CyTOF XT instrument.
Lists the standard reagents and consumables required for operation.
Identifies special tools and kits provided or recommended for the CyTOF XT.
Lists parts that can be ordered by customers for the CyTOF XT.
Lists consumables that are not supplied by Fluidigm but are required for the system.
Provides instructions for ordering consumables and reagents.
Details the directives and standards the CyTOF XT system complies with.
Lists general regulations and directives applicable to the instrument.
Lists harmonized standards used for safety and performance evaluation.
Explains the meaning of conformity markings found on the instrument.
Provides a general introduction to the CyTOF XT system and its capabilities.
Details the major modules and components of the CyTOF XT system.
Describes the Autosampler Module and its role in automated sample handling.
Explains the carousel's capacity, tube locations, and chilling features.
Details the function and operation of the Autosampler probe line.
Describes the wash tube's function in rinsing the probe line.
Explains the bottles in the station and their respective contents and functions.
Details the pump and valve unit, including syringe pumps and rotary valves.
Describes the function of the two syringes in sample introduction and wash.
Explains the roles of the selector valve and switching valve in fluidic control.
Covers the operational aspects of the Autosampler module.
Details the process of loading and injecting samples into the system.
Provides guidance on acquiring samples that are in pelleted form.
Offers recommendations for acquiring samples in suspension.
Explains the mixing function for pellet resuspension and sample loading.
Describes the probe line's stirring function to prevent sample settling.
Discusses the sample loop vibration setting for mitigating cell settling.
Details the automated clog detection and removal workflows.
Explains the function of the nebulizer assembly in sample aerosolization.
Describes the nebulizer rest and its use for storing the nebulizer.
Details the heater assembly, including the spray chamber and injector holder.
Explains the role of the spray chamber in heating and directing aerosol.
Describes the process of vaporizing, atomizing, and ionizing sample droplets.
Details the components and function of the plasma torch assembly.
Explains how aerosolized samples are ionized in the plasma.
Describes the vacuum interface's role in transporting ions to the ion optics.
Explains how high-pass ion optics remove unwanted low-mass ions.
Details the TOF mass analyzer's function in separating ions by mass-to-charge ratio.
Describes how events are detected and the format of the acquired data files.
Explains the discrete dynode electron multiplier detector and its operation.
Covers the process of acquiring and analyzing data, including dual count scale.
Explains the dual count scale method for quantifying bound antibody labels.
Diagram identifying the primary components of the CyTOF XT instrument.
Illustrates the status panel and explains the meaning of LED indicator colors.
Annotated figure showing the main components of the Autosampler Module.
Annotated figure of the Autosampler Module's pump and valve unit.
Annotated figure showing the Autosampler Module's probe line.
Annotated figures of the Autosampler Module's carousel housing and carousel.
Annotated figure of the Autosampler Module's nebulizer rest.
Annotated figure of the Autosampler Module's bottle station.
Annotated figures showing the nebulizer assembly and its expanded view.
Annotated figures of the heater assembly and its expanded view.
Annotated figures of the torch assembly and its expanded view.
Annotated figures showing the interface assembly and the torch box.
Details other system components like AC box, sampler cone, and skimmer-reducer.
Annotated images of the nebulizer and its components.
Annotated image of the nebulizer holder with the nebulizer attached.
Annotated image of the outer torch component.
Annotated image of the inner torch component.
Annotated image of the spray chamber component.
Annotated image of the injector holder component.
Annotated image of the injector component.
Annotated image of the injector cap component.
General guidelines and warnings for routine cleaning of the CyTOF XT system.
Procedure for cleaning the nebulizer, including daily and overnight methods.
Step-by-step instructions for safely removing the nebulizer assembly.
Detailed steps for daily cleaning of the nebulizer using Contrad/Decon.
Procedure for removing stubborn clogs using hot deionized water (DIW).
Instructions for cleaning and maintaining the nebulizer rest.
Guidelines for cleaning the injector and spray chamber components.
Steps for safely removing the heater assembly from the instrument.
Procedure for removing the injector cap and the injector.
Instructions for cleaning the injector component.
Steps for removing the spray chamber and injector holder.
Instructions for cleaning the spray chamber and injector holder.
Procedure for cleaning the vacuum interface cones (sampler, skimmer, reducer).
Step-by-step guide to removing the sampler and skimmer-reducer cones.
Instructions for cleaning the cones using Citranox and DIW, including sonication.
Detailed steps for reinstalling the skimmer-reducer and sampler cones.
Procedure for reassembling the injector and spray chamber components.
Instructions for replacing the O-ring on the nebulizer port.
Procedure for checking and replacing the O-ring on the nebulizer holder.
Steps for correctly reassembling the nebulizer and its components.
Procedure for weekly cleaning of fluidic lines and nebulizer using bleach solution.
A summary table of parts, cleaning frequency, agents, and procedures.
A checklist to ensure correct cleaning and reassembly of system components.
Procedures for maintaining the Autosampler module.
Instructions for replacing the sample loop in the Autosampler.
Steps for replacing the probe line on the Autosampler.
Procedure for recalibrating the probe line height after replacement.
Instructions for replacing a bottle line in the Autosampler.
Procedure for cleaning the Autosampler carousel.
Instructions for cleaning the bottle tray in the Autosampler.
Steps for replacing syringes in the Autosampler pump.
Procedures for maintaining the nebulizer assembly.
Instructions for replacing the nebulizer line.
Steps for replacing the nebulizer gas line.
Procedure for cleaning the nebulizer mount.
Procedure for removing and cleaning the torch assembly.
Steps for disassembling the torch assembly for cleaning.
Instructions for cleaning the inner and outer torch components.
Steps for reassembling the torch assembly after cleaning.
Procedure for filling the instrument's chiller with coolant.
Instructions for checking and replacing the cooling system air filter.
Procedure for checking and replacing the Autosampler air filter.
Troubleshooting steps for when the instrument's LED status panel is not lit.
Solutions for loss of or no communication between the instrument and computer.
Troubleshooting steps for no or poor vacuum readings.
Guidance for issues with plasma ignition or stability.
Troubleshooting for incorrect auxiliary and plasma gas flows or pressures.
Steps to check for and remove liquid from the spray chamber.
Guidance on checking and correcting torch alignment.
Troubleshooting steps for leaking syringes.
Addresses issues encountered during automated tuning processes.
Troubleshooting for XY alignment failures during tuning.
Guidance on optimizing detector voltage and troubleshooting out-of-range values.
Troubleshooting steps for current optimization, especially related to cone cleaning.
Troubleshooting for nebulizer gas flow or pressure issues.
Guidance for addressing issues with makeup gas flow.
Troubleshooting QC failures related to beads, sample introduction, and spray.
Addresses general tuning failures not covered elsewhere.
Troubleshooting for dirty, damaged, or improperly installed injectors.
Steps for identifying and addressing leaks within the Autosampler module.
Troubleshooting clogs or insufficient flow in fluidic lines and nebulizer.
Troubleshooting when insufficient sample is present.
Addresses poor event detection and antibody streaking in plots.
Guidance on dealing with high levels of environmental contamination.
Advice on diluting samples with excessively high cell concentrations.
Guidance on shutting down the instrument during scheduled power outages.
Step-by-step instructions for properly shutting down the CyTOF XT.
Instructions for restarting the CyTOF XT instrument after shutdown.
Recommendations for optimal performance, including annual service and part replacement.
Guidelines for cleaning and decontaminating the system before servicing or transport.
Procedure for cleaning surfaces using alcohol when handling biohazardous materials.
Information on the proper handling and disposal of used tubes and reagents.
General safety practices for laboratory and manufacturing areas.
Safety warnings related to instrument modification, biohazards, hot surfaces, and physical injury.
Explanation of hazard symbols used in the document and on the instrument labels.
Critical safety warnings and guidelines regarding electrical hazards.
Guidelines for safe handling, storage, and disposal of chemicals.
Information on ensuring adequate ventilation to mitigate health hazards.
Safety precautions for handling pressurized gas cylinders, especially argon.
Specific advice on the safe use, storage, and handling of gas cylinders.
Safety recommendations for handling laboratory reagents and consumables.
Warnings regarding high levels of RF energy and safety interlocks.
General| Brand | Fluidigm |
|---|---|
| Model | CyTOF XT |
| Category | Laboratory Equipment |
| Language | English |
To Next Page
