Appendix F: Safety
Pressurized Gas Safety
CyTOF XT User Guide 147
WARNING The instrument or system is designed for analysis of fixed/
permeabilized, non-live cells only. Under normal operation, cells are completely
combusted in the inductively coupled plasma. High levels of UV radiation inside
the torch box are significantly above lethal levels for most single airborne cells.
However, in the event of plasma shutdown, the non-ionized portion of a sample
can enter the torch box exhaust gases.
Pressurized Gas Safety
Safe Handling of Gas Cylinders
Argon gas used with the system is normally stored in liquid argon tanks or pressurized
containers. Carefully use, store, and handle compressed gases in cylinders. Gas cylinders
can be hazardous if they are mishandled. Argon is neither explosive nor combustible.
Contact the gas supplier for a safety data sheet containing detailed information on the
potential hazards associated with the gas.
IMPORTANT If liquid argon is used, the gas cylinder must be fitted with an overpressure
regulator, which vents the cylinder as necessary to prevent it from becoming a safety hazard.
WARNING Do not use electronic pressure regulator and auto switching valves
because doing so may affect the plasma stability and may result in frequent loss of
plasma.
WARNING It is recommended to install an oxygen sensor in the room where the
operator and gas storage are located.
Sample Handling and Preparation Safety
WARNING For better control of contamination, dedicate laboratory reagents and
consumables to use with CyTOF® instruments and Maxpar® reagents only.
Radio Frequency Radiation Safety
WARNING RADIO FREQUENCY RADIATION. The system generates high levels of
RF energy, which is potentially hazardous if allowed to escape. The system is
designed to contain the RF energy within the shielded enclosures of the torch
compartment and the RF power supply. Safety interlocks prevent the system from
operating without all covers, doors, and shields in place.
WARNING Inductively coupled plasma-based systems generate high levels of
radio frequency (RF) energy within the RF power supply and the torch box. RF
energy is potentially hazardous if allowed to escape. Do not bypass or disconnect
safety devices and safety interlocks.
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