Fluidigm Juno - Best Practices; Getting Started; Related Documentation; Workflow

Genotyping with Juno Getting Started Guide
7
Getting Started
Workflow
Best Practices
• Use good laboratory practices to minimize contamination of samples. Use a new
pipette tip for every new sample. Whenever possible, separate pre- and post-
PCR activities. Dedicate laboratory materials to designated areas.
• Unless otherwise specified, thaw reagents at room temperature, then use them
at room temperature. Store reagents at their specified storage temperatures.
(See “Required Kit Contents” on page 8.)
• Vortex reagents for 20 seconds, and then centrifuge reagents for 2 seconds
before use.
Related Documentation
Go to fluidigm.com/documents
Reagent Handling Automated Steps Estimated Times
1 Prepare preamplification and
genotyping assay and sample mixes
30–60 minutes
2 Pipet preamplification, genotyping
mixes, and control line fluid into the IFC
10–20 minutes
3
Run a script to preamplify and
genotype the DNA
2.5 hours (TaqMan
protocol); 3.5 hours
(SNP Type protocol)
4
Perform genotyping analysis on
EP1 or Biomark systems
5–10 minutes