Genotyping with Juno Getting Started Guide
Chapter 3: Genotyping with the Juno 96.96 Genotyping IFC Using SNP Type Assays
Perform Genotyping Analysis on the Samples
27
14 After the run is finished, tap EJECT to eject the IFC from the instrument.
IMPORTANT After a run, do not leave the IFC overnight in the instrument. Doing so
will adversely affect the reaction.
Perform Genotyping Analysis on the
Samples
Refer to the appropriate document:
• SNP Genotyping User Guide (PN 68000098)
• Biomark HD Data Collection User Guide (PN 100-2451)
• Biomark/EP1 Data Collection User Guide (PN 68000127).
Table 8 SNP Type genotyping
Cycles Temperature Time
Hot start 95 ºC 10 min
1
95 ºC 15 sec
64 ºC 45 sec
72 ºC 15 sec
1
95 ºC 15 sec
63 ºC 45 sec
72 ºC 15 sec
1
95 ºC 15 sec
62 ºC 45 sec
72 ºC 15 sec
1
95 ºC 15 sec
61 ºC 45 sec
72 ºC 15 sec
39
95 ºC 15 sec
60 ºC 45 sec
72 ºC 15 sec
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