Genotyping with Juno Getting Started Guide
Chapter 3: Genotyping with the Juno 96.96 Genotyping IFC Using SNP Type Assays
Load and Run the IFC on Juno
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7 Unseal the SNP Type assay plate and pipet 4.0 μL of each assay mix into an assay
inlet. (See the black circles on the pipetting map and “Prepare the Assay Mix” on
page 22.)
8 Unseal the sample plate and pipet 4.0 μL of each sample mix into a sample inlet.
(See the green circles on the pipetting map and “Prepare the Sample Mix” on
page 23.)
IMPORTANT Do not go past the first stop on the pipette. Doing so may introduce air
bubbles into the inlets. Pipet 4.0 μL from the 5.0 μL overage volume to ensure that
no air bubbles enter the inlet.
9 Pull the sticker front tab down and away from the IFC to gently peel off the loading
map. Do not invert the IFC.
10 If necessary, remove any bubbles from an IFC inlet by removing the contents by
pipette and then carefully re-pipetting the contents into the inlet.
11 Ensure that the SX interface plate (silver label) is installed in the instrument. [See the
Juno System User Guide (PN 100-7070).]
12 Place the IFC into the Juno instrument, then start the run <60 minutes after pipetting
the reagents into the IFC.
13 On Juno Scripts screen, tap the SNP Type tab, Juno 96.96, then Run. It takes
3 hours and 20 minutes to complete.
The script contains these thermal cycling protocols:
Table 7 Multiplex STA
Cycles Temperature Time
Hot start 95 ºC 2 min
14 95 ºC 15 sec
60 ºC 4 min
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