Fluidigm Juno - Page 25

To Next Page

To Previous Page

Genotyping with Juno Getting Started Guide

Chapter 3: Genotyping with the Juno 96.96 Genotyping IFC Using SNP Type Assays

Load and Run the IFC on Juno

2 Review the pipetting map, which provides specific instructions for pipetting reagents

in the IFC. Pipet reagents from the SNP Type assay plate and the sample plate to the

IFC. On the pipetting map, each inlet is labeled with the plate well location of the

sample or assay to be pipetted into that inlet:

3 Ensure that the notched corner of the IFC (“A1”) is at the top left.

4 Load an entire syringe of Juno 96.96 GT Control Line Fluid in Acc1 and a second

syringe in Acc2. (See the pink squares on the pipetting map.) To ensure correct

accumulator volume, only use syringes containing Juno 96.96 GT Control Line Fluid.

5 Load an entire syringe of Juno 96.96 GT Control Line Fluid into each of the two

reservoirs. (See the long pink rectangles on the right side of the pipetting map.)

IMPORTANT Carefully dispense control line fluid into the reservoirs. If control line

fluid comes into contact with the sample inlets, use a new IFC.

6 Pipet 15 μL of Juno GT Flux Fluid into each of the six ports. (See the purple circles on

the pipetting map.)

Key

Load 1 Load 2

Juno 96.96 GT Control

Line Fluid

Assay mix, 4.0 μL

Juno GT Flux Fluid,

15 μL

Sample mix, 4.0 μL

Juno 96.96 GT Control

Line Fluid

—

Empty

Acc 1

Acc 2

A01

A02 A03 A04 A05

A06

A07

A08 A09 A10 A11

A12

B01

B02 B03 B04 B05

B06

B07

B08 B09 B10 B11

B12

C01

C02 C03 C04 C05

C06

C07 C08 C09 C10 C11

C12

D01 D02 D03 D04 D05

D06

D07 D08 D09 D10 D11

D12

E01

E02 E03 E04 E05 E06

E07

E08 E09 E10 E11 E12

F01 F02 F03

F04 F05

F06

F07 F08 F09 F10 F11

F12

G01

G02 G03 G04 G05

G06

G07

G08 G09 G10 G11

G12

H01

H02 H03

H04 H05

H06

H07

H08 H09

H10 H11

H12

A01