Genotyping with Juno Getting Started Guide
4
About This Guide
CAUTION ABBREVIATED SAFETY ALERTS. Hazard symbols and hazard
types specified in procedures may be abbreviated in this document. For
complete safety information, see the safety appendix on page 30.
Purpose
This guide describes how to perform genotyping of low-concentration DNA with
the Juno
™
96.96 Genotyping IFC (integrated fluidic circuit) on the Juno™ system.
This is possible through advanced microfluidics technology that integrates
preamplification and genotyping reactions of up to 96 samples and 96
genotyping assays in a single workflow on an IFC.
The IFC produces 9,216 genotypes in less than three hours using a simple
workflow with minimal hands-on time. Samples are loaded into individual inlets of
the Juno 96.96 Genotyping IFC, then distributed across multiple reaction
chambers in nanoliter-volume aliquots. With high-quality samples, detecting the
specific targets requires thermal cycling for preamplification and PCR for
genotyping on the instrument.
After genotyping is performed on the Juno system, the IFC is scanned on the
EP1
™
system or the Biomark
™
HD system to collect genotyping data for later
analysis.
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