29-0047-78 AC 04-2014
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All spectrophotometers are operated in the same generic way by comparing the
amount of light Absorbed by a sample when compared to a reference solution:
2.1. Preparation before starting
• Theinstrumentisswitchedonandallowedtoinitialise,Ultrospec10,
NovaspecIII/Plus,GeneQuant100/1300,SimpliNanoandNanoVuePlusunitshave
the on/off switch on the keypad.
• Ultrospec2100unitshaveanon/offswitchonthebackoftheinstrumentonthe
right hand side.
• Ultrospec7000/8000/9000instrumentshavetheon/offswitchonthelefthand
side of the instrument.
• Therelevantsoftwaremodulefortheapplicationbeingcarriedoutisselectedfrom
within the instrument software e.g. fixed wavelength measurement, concentration
measurement, wavelength scan, kinetics measurement or a dedicated method
such as DNA concentration & purity. The options within the application set up the
instrument parameters such as wavelength and define any calculations taking
place on the measured results.
2.2. Performing a run - cuvettes
• OntheUltrospec10,NovaspecIII/Plus,GeneQuant100/1300andUltrospec
2100/7000/8000/9000 units, samples are measured in a cuvette. A suitable
cuvette must be used for the application to be carried out – it must be of the
correct material to allow the selected wavelength of light to pass through and
must be of the correct physical size to fit the relevant instrument and the sample
volume available. The cuvette is filled with a solution of reference material which
is typically the solution that the sample is dissolved in, the cuvette is placed in the
cell holder in the correct orientation as per the diagrams below:
Ultrospec 10, front to back All other units, right to left
• TheinstrumentisthenzeroedbypressingtheZerokeyonthekeypad:
2. OPERATING AND
MAINTENANCE
Ultrospec10NovaspecIII/PlusGeneQuant100/1300Ultrospec2100