Center the Beam (if the beam is not centered)
Push the “BH” button, adjust the Beam centre by using the X, Y knobs on the operation panel.
Start acquiring TEM images (Please always keep attention to the exposure intensity)
(a) Find your sample using the Screen camera (Left display)
You can use HC (>0.2K) mode to search your sample at the beginning, then change to HR mode (> 4 K) for
getting images
(b) Lower the beam intensity and click “Scr. out”
(c) Open the “Gatan Digital Micrograph” software (Right display)
(d) Click “Start View” to start the image acquisition
Put the cursor in the blank area of the image window, adjust the intensity 2,000~3,000.
(e) Use “Focus” on the operation panel to adjust the focus of the sample
(f) Click “Start Acquire” to record the final image
(g) Click “File” –> “Save Display As” to save your image
(h) Switch to Screen camera: Click “Stop View”, then “Scr. in” (left display), double click
“Link Screen”.
Before changing samples, please reduce magnification to 4 K and set the spacemen stage at the home
position (x, y, α = 0)
Session shut down:
o Reduce magnification to 4 K
o Set the spacemen stage at the home position (x, y, α = 0)
o Remove the sample from the specimen holder, and store the holder in the standby position
o Click “Beam off” to turn off the beam
o Stop the Screen camera (if there are no users right after you)
o Click “Film off” to turn off the filament voltage
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