20
Preparing Samples for Use on the Catalyst One Analyzer
You can run untreated whole blood, lithium heparinized whole blood, plasma, serum, and urine
samples on the Catalyst One analyzer.
IMPORTANT: Do not use EDTA or sodium heparin for chemistry analysis.
To Prepare an Untreated Whole Blood Sample
(Using a Lithium Heparin Whole Blood Separator)
1. Remove the green cap from the lithium heparin whole blood separator to prepare it for
sample collection.
2. Immediately after sample collection (to avoid clotting), dispense 0.6–0.8 cc of untreated (no
additive) whole blood into the lithium heparin whole blood separator using an untreated syringe
with the needle removed.
Tip: Use the fill line on the separator to ensure proper fill volume.
Note: Heparinized samples can be used in the lithium heparin whole blood separator except
when running feline AST, LDH, or CK. Double dosing may elevate the results for these assays in
feline samples.
3. Gently swirl (do not invert or shake) the whole blood separator at least 5 times to mix the
sample with the anticoagulant.
Caution: Ensure that the cap is removed before loading the separator into the
analyzer.
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32
Fill to lowest line on
separator (0.7 cc [700 µL])
To Prepare a Plasma Sample
1. Use the appropriate tube and collection device.
2. Draw the sample gently and transfer if necessary.
Note: Be sure to use the correct blood-to-lithium heparin ratio.
3. Gently invert (do not shake) the sample for 30 seconds to mix.
4. Centrifuge the sample.
5. Use a transfer pipette (or a 300 µL pipette) to transfer the appropriate volume of sample to a
Catalyst sample cup (ensure there are no bubbles in the sample cup). The volume needed
varies depending on the number of slides being used in the run—for more information, see
“Proper Sample Cup Volume” on page 22.
Sample Preparation and Storage
2 3 4 5
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