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Complementary Tests
Complete urinalysis with culture and sensitivity. Serum chemistries such as creatinine, BUN,
albumin, and globulin.
CBC
SNAP 4Dx Test
Storage Information
Handle and store urine samples in closed containers to avoid evaporation and contamination.
Samples may be stored at room temperature for up to 4 hours. Refrigerated samples may be stored
up to 3 days. Do not freeze samples.
Do not use hemolyzed specimens as hemoglobin increases results significantly. Intact red blood
cells can be removed via centrifugation.
Reaction Sequence
Medical Protocol Descriptions
Ammonia Protocol
Baseline ammonia levels should be assessed in animals with signs of hepatic encephalopathy
or in patients suspected of having portosystemic shunts (PSS). Ammonia tolerance tests may be
considered to evaluate for PSS where bile acids are not considered (for example, in Maltese).
Ammonia tolerance test: A baseline sample is drawn after the patient has been fasted for
12 hours. Ammonium chloride (0.1 g/kg) by mouth via stomach tube or gelatin capsules.
A second sample is drawn 30 minutes after ammonium chloride administration.
Note: Vomiting during the procedure will invalidate results.
Sample Requirements: 1 mL heparinized plasma, separated from RBCs. Do not use serum.
Storage/Stability: 48 hours—store plasma frozen
Interferences: Hemolysis, glucose levels over 600 mg/dL (33.33 mmol/L), high BUN values
Comments: Anticoagulated blood must be centrifuged immediately after collection. Separate
plasma and place it in a glass container (RTT). Freeze immediately and keep frozen if not running
sample immediately.
Note: Ammonia levels increase with time.
UPC Protocol
Principle Reason for Performing Test: To aid in the diagnosis of protein-losing nephropathies
such as glomerulonephritis and amyloidosis and as an early marker of chronic renal failure.
Includes: Urine protein (UPRO), urine creatinine (UCRE), protein:creatinine (UPC) ratio
Sample Requirements: 2 mL urine in a sterile container
Storage/Stability: 48 hours at 2°C–8°C (36°F–46°F)
Interferences: Gross hematuria, pyuria.
Complementary Tests: Complete urinalysis with culture and sensitivity. Serum chemistries such
as creatinine, BUN, albumin, globulin; CBC; SNAP* 4Dx* Test; and imaging studies.
Appendices
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