31
Troubleshooting, Continued
Low Reading
Your sample is out of range. Use a sample that is more
concentrated or use a lower dilution (i.e., 20 L in 180 L
instead of 10 L in 190 L).
View the Check Standards Screen to confirm that the values
for the samples fall between the values of the standards (see
page 18).
Ensure that you have prepared the Qubit
™
working solution
correctly (1:200 dilution using the buffer provided in the kit).
Ensure that you have prepared the standard tubes correctly
(10 L of each standard in 190 L of Qubit
™
working solution).
Ensure that the standard and sample tubes are filled to 200 L.
Protect the Qubit
™
reagent and working solutions from light.
Select the correct Qubit
®
2.0 Fluorometer assay for the Qubit
™
assay you are performing and calibrate the fluorometer
correctly. Standards must be used in the correct order.
Ensure that the assay is performed entirely at room
temperature.
Continued on next page
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