Leica DMi8 User Manual

10
Acquisition Window
Select Z for Z-stack and T for time series.
Mark and Find
Tile Scan
FIM: fluorescence intensity manager, higher % more light
IL – FLD: field stop, should always be at 6
Camera %: (light is directed towards eyepiece) or 100 (light is
directed to camera)
Change objective here
Keep “Magnification Changer” as “ 1 “
Binning: select if you want a faster, quicker image with less
resolution exposure here.
Longer exposure = more light = higher chance of photobleaching
Binning: increases exposure but decreases resolution
Scroll through the Z to find when your subject just begins to dim and
go out of focus, then press BEGIN. Go through the focus to the next
point the subject starts to go out of focus and click END.
The system will provide number of steps with “optimized” Z-step
size if “System Optimized” box is checked. User can assign step size
or number of steps with corresponding box checked.

Leica DMi8 Specifications

General

Type	Inverted Microscope
Software	Leica Application Suite X (LAS X)
Dimensions	Varies depending on configuration
Weight	Varies depending on configuration
Imaging Modes	Brightfield, Phase Contrast, DIC, Fluorescence
Objective Compatibility	Leica Objectives (various threads depending on nosepiece)
Stage	Motorized XY stage
Camera Compatibility	Leica cameras and 3rd party cameras via C-mount or direct camera ports
Illumination	LED illumination
Focusing	Motorized Z-drive
Nosepiece	Motorized 6-position nosepiece
Automation	Fully automated
Optical System	Infinity Optical System
Magnification Range	Varies depending on objective used
Observation Tubes	Various ergonomic observation tubes available (binocular, trinocular)
Filter Slots	Multiple filter cube slots
Camera Port	C-mount
Software Compatibility	Compatible with Leica LAS X software