LEITZ LABORLUX S - Phase Contrast Objectives; Phase Contrast with the UKL Universal Condenser

16
Annular
sto p
EF objectives
PLAN objectives
I
10/0.25 PHACO I PLAN 10/0.25
PHACO I
2
25/0.40 PHACO 2
PLAN
20/0.40
PHACO I
40/0.65 PHACO 2
PLAN
40/0.65
PHAC02
3
10011.25 PHACO 3
PLAN
10011.25 OEL PHACO 3
Phase conlr.lsl objectives
Phase contrast with the UKL universal condenser
[nsert the UKL condenser into the condenser mount (1.9) and
bring to its highest position with the vertical adjustm en t (1.8).
Set the
aperture diaphragm (9.5) to "10" (green). Put a speci-
men on the stage. Turn in the 10/0.25 PH ACO I ob j ec tive.
Set the annu lar stop turret at position I and focus (he spec imen
with the coa rse and fine drive. Cl ose th e field diaphragm. Set
Ihe condenser with the vertical adjustment (1.8) so that the
ed ges of th e clo sed fi e ld diaphragm are in sharp foclis. Centre
the image
of the field diaphragm with th e two centdng screws.
Open the tield diaphragm until it ju st dis appe ars from the field
of view. Remove an eyepiece from the e yepiec e tube and insert
the focus
in g telescope. Loo sen t he clamp ring on th e focusing
telescope and adjust the eyel ens until li ght and pha se rings are
in sharp focus. Using the cenlring screws (press in a nd turn)
adjust the lig ht ring so that it is exac tly covered by the phase
ring of the objective. Ce nlralion must be c<:Irried out on ce for
all
objective/annul ar stop combina tions <:Ind is th en retained
for all further settings.
Fig. 17 Phase ring and annular stop as s een through the fo cusing
telescope.
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