Making Measurements
Operational Hints
4-48 Using the LI-6400
4
1 Use the 6400-01 CO
2
Mixer
All the incoming air is scrubbed by the soda lime column, and the mixer adds
whatever CO
2
is necessary to hold your requested concentration.
If you are using the mixer, youÕll need to connect your CO
2
source (a CO
2
cartridge or tank - see 6400-01 CO2 Injector Installation on page 2-7) about
5 or 10 minutes before you expect to use the system, and let it pressurize the
internal workings of the mixer.
2 Use a buffer volume
When air is moved through a large, mixed volume, fluctuations in incoming
CO
2
are greatly damped, and can be stable enough to use for gas exchange
purposes (Figure 4-12).
Acceptable volumes depend on the magnitude of the fluctuations that need to
be damped, but several liters is a good starting volume. A plastic five gallon
enclosed bucket is a good buffer volume, or - if nothing else - use the LI-6400
carrying case as a buffer volume.
3 Use tank air, or some other source
An advantage of an open system is that you can condition the incoming air
stream, using CO
2
tanks, humidifiers, oxygen generators, etc., prior to intro-
ducing that air to the leaf chamber.
Figure 4-12. A buffer volume will dampen fluctuations in concentration.
Time
CO
2
Time
CO
2
To LI-6400
INLET
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