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Millipore Sigma Scepter 3.0 - User Manual

Millipore Sigma Scepter 3.0
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The life science business of Merck operates
as MilliporeSigma in the U.S. and Canada.
The life science business of
Merck KGaA, Darmstadt, Germany
operates as MilliporeSigma
in the US and Canada.
SigmaAldrich.com
User Guide
Scepter™ 3.0
Handheld
Automated
Cell Counter
PHCC30000
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Summary

Introduction to Scepter 3.0

Safety and Precautions

General Warnings

Important safety warnings and hazard information for using the instrument.

Battery Safety

Specific safety guidelines and warnings related to the instrument's battery.

Parts and Functions Overview

Instrument Features

Details on the display screen, eject button, sensor port, and disposable sensor.

Controls and Icons

Explanation of the instrument's control buttons, screen icons, and indicators.

Battery and Charging

Charging Station Installation

Getting Started with Scepter 3.0

Date and Time Setup

Guide to setting the date and time on the Scepter 3.0 instrument.

Bluetooth Printer Connectivity

Steps for pairing the instrument with a Bluetooth-enabled printer.

Wi-Fi Network Setup

USB Drive Formatting

Instructions for formatting a USB drive for Wi-Fi configuration files.

Create Wi-Fi Configuration File

Steps to create a configuration file for connecting to a Wi-Fi network.

Connect to Wi-Fi Network

Procedure for connecting the Scepter 3.0 to a Wi-Fi network.

Gating Methods

Auto Gating Method

Using the automatic gate setting based on histogram profile.

Use Last Gating Method

Maintaining previously set gating parameters for analysis.

Display Unit Settings

Sensors and Usage

Sensor Technology

Explanation of the microfluidic sensor technology and Coulter Principle.

Attaching the Sensor

Step-by-step guide for correctly attaching the Scepter 3.0 sensor.

Cell Counting Procedure

Sample Preparation and Diluents

Guidelines for sample dilution and recommended diluting solutions.

Using Test Beads

Instructions for using Scepter 3.0 Test Beads for system verification.

Counting Process Execution

Histogram Explorer

Navigating and viewing saved histograms from the counting process.

Edit Analysis Parameters

Adjusting low and high gates for histogram analysis.

Switch Volume;Diameter View

Changing the histogram X-axis display between volume and diameter.

Scale Y-Axis Count

Adjusting the Y-axis scale for count representation on the histogram.

Data Export Options

Export to USB Drive

Steps to export histogram data to a USB thumb drive.

Export via Wi-Fi Network

Downloading test data wirelessly to a computer via Wi-Fi.

Reviewing and Deleting Data

Deleting Saved Data

Procedure for removing histograms from the instrument's memory.

Printing Histograms

Help Screens and Tutorials

Accessing built-in help topics and tutorials for instrument usage.

Network Information (IP, DNS, MAC)

Retrieving network details like IP address for data upload.

Firmware Updates

Information on how to update the Scepter 3.0 instrument firmware.

Storage and Maintenance

Storage Conditions

Recommended environmental conditions for storing the instrument.

Cleaning and Sanitizing

Guidelines for cleaning and sanitizing the instrument and its components.

Troubleshooting Guide

Instrument Error Messages

Technical Specifications

Contact Information

Information on where to find office locations for assistance.

Technical Assistance

Guidance on how to obtain technical support for the instrument.

Standard Warranty

Information regarding the product's warranty terms and conditions.

Product Ordering Information

Related Products and Accessories

Global Registration Marks

Millipore Sigma Scepter 3.0 Specifications

General IconGeneral
BrandMillipore Sigma
ModelScepter 3.0
CategoryLaboratory Equipment
LanguageEnglish