Millipore Millicell ERS-2 User Manual

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User Guide
Millicell
®
ERS-2
Electrical Resistance System
MERS00002
Introduction
The Millicell
®
ERS-2 Electrical Resistance System is
a meter and electrode system designed to reliably
measure Trans Epithelial Electrical Resistance (TEER)
of epithelial cells in culture. An increase in TEER
detected with the electronic circuit of the Millicell
®
ERS-2 meter and its electrode is an indication of cell
monolayer health and confluence.
A silver/silver chloride (Ag/AgCl) pellet on each
electrode tip measures voltage. The small size of
the electrode enables the user to easily measure
transepithelial voltage and the resistance of cells
grown on microporous membranes.
This system is for research use only.
Benefits of AC Power Source
Over DC Power Source
The Millicell
®
ERS-2 system uses alternating current
(AC) to make membrane resistance measurements.
Using an isolated AC source has several advantages
over the traditional use of direct current (DC):
• Membrane voltage and voltage electrode offset
do not affect resistance measurements.
• The zero net charge on the cells eliminates the
adverse effects of DC current on the cell membrane.
• There is no electrochemical deposition
of electrode metals.
• Membrane capacitance does not affect resistance
readings. Once the Millicell
®
ERS-2 system is
standardized, you can use it to quantitatively
measure cell confluence.
The Millicell
®
ERS-2 System
and Components
The Millicell
®
ERS-2 system consists of a meter,
STX01 electrode, STX04 1,000 Ω test electrode,
and battery charger.
Analog output
Digital LCD display
Mode Switch
Input Port
R Adj Calibration
Power Switch
STX01 Electrode
STX04 Test
Electrode
The Meter
The unit measures 18.4 cm × 10.8 cm × 5.8 cm
and includes an internal rechargeable 6 V NiMH
2,700 mAh battery pack with an external 12 V DC
battery charger, equipped with an AC power cord.
An analog output port allows output of analog data
to a recording device.
The Electrode
The electrode is connected to a wire with
a telephone-type plug at its end. This plug connects
to the Input port at the front of the meter during use.

Millipore Millicell ERS-2 Specifications

General

Model	Millicell ERS-2
Accuracy	±1% of reading
Display	Digital LCD
Power Supply	9V battery or AC adapter
Electrode Material	Ag/AgCl
Electrode Compatibility	Compatible with Millicell culture plate inserts
Relative Humidity	20% to 80% non-condensing
Measurement Range	0.1 Ω·cm² to 100 kΩ·cm²

Summary

Millicell ERS-2 System Overview

Introduction to TEER Measurement

Explains TEER measurement using the Millicell ERS-2 system for cell health and confluence.

Advantages of AC Power Source

Details the benefits of using AC power for membrane resistance measurements over DC.

Millicell ERS-2 System Components

Lists the meter, STX01 electrode, STX04 electrode, and battery charger.

Millicell ERS-2 Meter Description

Describes the physical dimensions, battery, and output port of the Millicell ERS-2 meter.

Millicell ERS-2 Electrode Description

Describes the electrode connection via a telephone-type plug to the meter's input port.

System Installation and Testing

Symbols Used in User Guide

Explains symbols used throughout the user guide for safety and product information.

System Installation and Charging

Instructions for unpacking, installing power, and charging the system battery overnight.

System Functionality Testing

Describes periodic testing for proper operation and checks to be performed before each use.

Meter Functionality Check

Steps to check meter functionality by connecting the STX04 electrode and setting it to 1000 Ω.

Electrode Preparation and Measurement Setup

Electrode Equilibration for Voltage Measurements

Explains the need for STX01 electrode equilibration for accurate voltage measurements.

Electrode Functionality Check for Voltage

Steps to check electrode functionality for voltage measurements using the Millivolts mode.

Electrode Sterilization and Disinfection

Procedures for sterilizing or sanitizing/disinfecting the electrode before use.

Measuring Cell Resistance

Determining Blank Resistance

Instructions to measure resistance across a blank cup (insert without cells).

Resistance Calculations and Unit Area

Explains tissue resistance, unit area resistance, and provides a table of membrane areas.

Example Application: Measuring Resistance

An example application demonstrating measuring cell resistance in a 24-well plate.

Measuring Cell Voltage

System Maintenance and Storage

Guidelines for maintaining and storing the meter and system.

Changing the Battery Pack

Detailed instructions on how to replace the rechargeable battery pack.

Electrode Care and Troubleshooting

Electrode Storage Guidelines

Instructions for short-term and long-term storage of the electrode.

Electrode Cleaning Procedures

Steps for cleaning the electrode to maintain performance, including Tergazyme use.

Sanding Electrode Tips for Voltage

Instructions on lightly sanding voltage electrodes to improve readings.

Troubleshooting Common Issues

Lists common symptoms, causes, and corrective actions for system problems.

Troubleshooting Symptom-Cause-Action

Millicell ERS-2 Meter Specifications

Regulatory Compliance Information

Information on product conformity with European directives and safety standards.

Ordering Information and Components

Other Millicell Products

Lists other Millicell products, specifically single-well standing inserts.

Millicell Plate Assemblies and Accessories

Millicell 24-Well Plate Assemblies

Details of 24-well cell culture plate assemblies and their components.

Millicell 96-Well Plate Assemblies

Details of 96-well cell culture plate assemblies and their components.

System Accessories

Lists available accessories for the Millicell system.

Important Notices and Disclaimers

Provides information and advice disclaimers, and subject to change notices.