Operation Manual____________________________________________________________Applications
_____________________________________________________________________________________________
12-2
PROCESSING DIFFICULT CELLS
When processing difficult cells, the use of enzymes should be considered. Glusulase
can be used with yeast, lysostaphin can be used with staphylococcus, collagenase can
be used with skin and cartilage, and trypsin hyaluronidase can be used with liver and
kidney. Glass beads in the 10 to 50 micron size range can also be added to the liquid to
expedite processing. When using glass beads, a good ratio is beads, liquid. Glass
beads concentrate the energy released by the implosion and facilitate the crushing of
cell walls. After processing, the glass will sink to the bottom of the vessel or can be
centrifuged or filtered out.
For further application information see section 16 (Literature Request).
To Next Page
Loading...