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Molecular Devices SpectraMax M5 User Manual

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SpectraMax
®
M3, M4, M5, and M5
e
Multi-Mode Microplate Readers
User Guide
0112-0115 F
July 2010

Table of Contents

Questions and Answers:

Molecular Devices SpectraMax M5 Specifications

General IconGeneral
Wavelength Range200 - 1000 nm
Light SourceXenon flash lamp
SoftwareSoftMax Pro Software
Wavelength Range (Fluorescence)250 - 850 nm
Wavelength SelectionMonochromator
ShakingLinear, Orbital, and Double Orbital
Detection ModesAbsorbance, Fluorescence, Luminescence
Plate Formats6- to 384-well plates
Temperature Control4°C above ambient to 45°C
Data InterfaceUSB

Summary

SpectraMax Reader Description

PathCheck Pathlength Technology

Explains how the technology normalizes well volumes to 1-cm cuvette readings.

Computer Control with SoftMax Pro

Explains control via SoftMax Pro Software for instrument functions.

Control Panel Overview

Details the LCD display and membrane keys for instrument control.

Microplate Drawer Operation

Describes the location, function, and operation of the microplate drawer.

Cuvette Chamber and Handling

Discusses the cuvette chamber and proper cuvette use and alignment.

Back Panel Components

Details components on the back panel: power switch, ports, fuse box, label.

Principles of Operation

Absorbance and Transmittance

Explains absorbance measurement (A = -log(I/Io)) and transmittance (T = I/Io).

PathCheck Pathlength Measurement

Explains the Beer-Lambert law and absorbance proportionality to pathlength.

PathCheck Interference Handling

Identifies turbidity and sample color as common interferences for PathCheck.

Time-resolved Fluorescence (TRF)

Details TRF mode, its advantage over fluorescence intensity, and detection parameters.

Fluorescence Polarization (FP)

Explains FP mode, data sets, and calculation of polarization (mP) and anisotropy (r).

Luminescence Principles

Describes luminescence as light emission from non-thermal processes.

Instrument Installation

Important Safety Precautions

Covers power safety, environment, temperature, and instrument handling warnings.

Setting Up the Instrument

Step-by-step guide for placing, connecting, and powering the instrument.

Instrument Operation

Optimizing Fluorescence Assays

Discusses adjusting wavelengths, plate blanks, and PathCheck for fluorescence.

Optimizing Absorbance Assays

Details factors for optimization like wavelengths, filters, PMT voltage, and Stokes' shift.

Instrument Maintenance

General Safety Warnings

Covers power, environment, electrical shock, optics handling, and organic solvent precautions.

Troubleshooting Guide

Manual Drawer Opening

Procedure to manually open the microplate drawer if it fails to open.

Understanding Error Codes

Lists error code ranges and their general causes.

Error Message Types

Differentiates between Fatal and Warning messages.

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