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QUICK GUIDE
Illustration xx A/B: Adjust the aperture diaphragm.
A
Aperture diaphragm handwheel
The zoom unit contains an aperture dia-
phragm. The aperture diaphragm can be
adjusted with the help of the aperture
diaphragm handwheel (1).
NOTE: Adjust the aperture dia-
phragm opening according to the
characteristics of the specimen.
B
Aperture diaphragm open/closed
OPEN CLOSED
Focus depth Shallow Deep
Brightness Bright Dark
Resolution High Low
Position of the aperture diaphragm
Opening and closing the aperture dia-
phragm causes a change in focus depth,
brightness and resolution.
c) Contrast adjustment by means of aperture diaphragm
(condenser diaphragm)
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QUICK GUIDE
7
Further adjustment options on the stand base
A
3
2
1
Illustration xx/A: OIC illumination.
OIC adjustment slider
The stand base (1) supports OIC illumina-
tion.
OIC means oblique coherent contrast il
-
lumination. An illumination method in
which contrast gain is achieved by par-
tial or complete coverage of the normal
illumination beam path. For this purpose,
a black OIC plate (2) is brought into the
beam path that can be moved via the OIC
adjustment slider (3) on the right-hand
side of the stand base (1). The specimen
is then radiated through at an angle, re-
sulting in a shadow cast on the ne struc-
tures of the preparation. This enables
the observation of colorless, transparent
samples structures with relief and high
contrast.
a) OIC (oblique coherent contrast) illumination
B
42
Illustration xx/B: OIC illumination settings.
3
OIC illumination settings
Move the OIC adjustment slider (3) along
the scale (4). You can observe how the
black OIC plate (2) moves into the beam
path.
Adjust until the optimum observation
condition is reached in combination with
the selected magnication and the sam-
ple to be examined.
• OIC plate completely out of the
beam path
(OIC adjustment slider either at the
furthest or closest position on the
scale)
No contrast and relief enhancement
visible
• OIC plate partially in the beam path
Contrast and relief enhancement of
colourless and transparent sample struc-
tures visible
• OIC plate completely in the beam
path
(OIC adjustment slider in the middle
position of the scale)
Completely darkened beam path
Helpful: Thanks to the gradua-
tion on the scale, you can save and
reproduce the desired lighting set-
ting.
1