Nikon Eclipse Ci-E

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Chapter 1 Microscopy Procedures

Chap. 1-2

Microscopy Procedures Phase Contrast Microscopy

OFF

TOGGLE

PATTERN

MEMORY

[Remote control pad]

Nosepiece address

buttons

Setting mode switch

Toggle pattern switch

Main Page

Introduction3
Contents of the Manual4
Symbols Used in this Manual4
Table of Chapters5
Table of Contents6
Safety Precautions9
WARNING and CAUTION Symbols9
Meaning of Symbols Used on the Product9
Warning9
Caution9
Notes on Handling the Product12
Microscopy Procedures13
Bright-Field Microscopy13
System Configuration and Controls13
Bright-Field Microscopy Procedure15
Phase Contrast Microscopy23
System Components and Controls23
Phase Contrast Microscopy Procedure25
Simple Polarizing Microscopy30
System Configuration and Controls30
Simple Polarizing Microscopy Procedure32
Sensitive Polarization Microscopy37
System Configuration and Controls37
Sensitive Polarization Microscopy Procedure39
Epi-Fluorescence Microscopy44
System Configuration and Controls44
Epi-Fluorescence Microscopy Procedure46
Individual Operations51
Adjusting the Brightness of a Diascopic Image51
Adjustment with the Dia-Illumination Brightness Control Knob51
Adjustment with ND Filters51
Focusing on the Specimen (Vertical Stage Movement)52
Focus Knob Rotation and Stage Movement53
Number of Focus Knob Turns and Distance of Stage Travel53
Adjusting the Rotating Torque of the Coarse Focus Knob54
Refocusing54
Position Exchange of the Fine Focus Knob55
Bringing the Specimen into the Optical Path (Horizontal Stage Movement)56
Knob Rotation and Stage Movement56
Adjusting the Knob Heights56
Adjusting the Knob Rotation Torque56
Adjusting the Diopter57
Focusing and Centering the Condenser58
Adjusting the Aperture Diaphragm59
Adjusting the Aperture Diaphragm Using the Condenser Scale59
Adjusting the Aperture Diaphragm Using the Centering Telescope59
Selecting a Condenser60
Adjusting the Field Diaphragm60
Switching the Optical Path of the Tube61
Light Distribution61
Disabling the Clicking of the Optical Path Switching61
Adjusting the Binocular Section62
Adjusting with the Binocular Section of the Tube62
Using the Eyelevel Riser62
Using at Lowered Position of Stage (Spacer for Nosepiece)62
Switching Objectives63
Basic Operation63
Switching between Two Objectives (Toggle)64
Configuring the Motorized Functions66
Configuring the Motorized Swing-Out Condenser66
Changing the Initial Brightness of Dia-Illumination LED67
Reverse Rotation Lock (Rotation from Address 1 to 6)68
Oil Immersion69
Water Immersion70
Tips for Phase Contrast Microscopy70
Tips for Epi-Fluorescence Microscopy72
Switching Excitation Methods73
Selecting Filters74
Capturing Images76
Photomicroscopy76
Tips on Microscope Settings for Photomicroscopy77
Assembly79
ECLIPSE CI-E System Configuration79
Assembly for Bright-Field Microscopy80
Assembly for Phase Contrast Microscopy85
Assembly for the Simple Polarizing Microscopy85
Assembly for Sensitive Polarization Microscopy86
Assembly for Epi-Fluorescence Microscopy87
Attaching a Camera89
Troubleshooting91
Optical System and Operation91
General91
Epi-Fluorescence Microscopy95
Phase Contrast Microscopy96
Electrical System97
General97
Epi-Fluorescence Microscopy98
Maintenance and Storage99
Cleaning99
Cleaning Lenses99
Cleaning Parts Other than the Lens99
Cleaning Immersion Oil100
Decontaminating the Product100
Storage100
Regular Inspections (Charged)100
Specifications and Safety Standards101
Microscopy (Principles)101
Performance Properties101
Physical Properties102

Nikon Eclipse Ci-E - Page 24

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