Nikon Eclipse Ci-E - Page 33

Chapter 1 Microscopy Procedures
21
Microscopy Procedures
Simple Polarizing Microscopy
Chap. 1-3
3
Set the setting mode switch to the left side (operation mode).
4
Turn on the dia-illumination LED.
5
Lower the condenser slightly from the uppermost position.
6
Fully open the field diaphragm and aperture diaphragm.
7
Bring the 10x objective into the optical path.
8
Place a specimen on the stage, and move the stage to bring the target into view.
9
Remove the analyzer and the polarizer from the optical path.
Pull out the analyzer IN/OUT knob from the analyzer tube
for simple polarization to remove the analyzer from the
optical path.
The polarizer unit for simple polarization has not yet been
installed at this point.
Removing the analyzer from the optical path
10
Focus on the specimen. (→See Chapter 2 “2 Focusing on the Specimen (Vertical Stage Movement)” for
details)
11
Adjust the diopter. (→See Chapter 2, “4 Adjusting the Diopter” for details)
12
Adjust the interpupillary distance.
13
Focus and center the condenser. (→See Chapter 2, “5 Focusing and Centering the Condenser” for details)