Nikon Eclipse Ci-E - Page 47

Chapter 1 Microscopy Procedures
35
Microscopy Procedures
Epi-fluorescence Microscopy
Chap. 1-5
2
Close the shutter and block the illumination path.
Set the shutter open/close lever of the epi-fluorescence
attachment to position “C” to close the shutter and block
the optical path.
A1
-
2
-
3
-
4
B 1
-
2 / 3
-
4
C1
-
2
-
3
-
4
1
1
4
2 3 4
CUBE
Closing the shutter
Bringing the filter cube into the optical path
Shutter of the epi-fluorescence attachment
The shutter blocks illumination.
If the specimen is continuously exposed to the strong
light of the mercury lamp, it may become damaged or
decolorized.
Be sure to close the shutter when suspending the
microscopy or when pausing epi-fluorescence
microscopy to perform microscopy with diascopic
light. Be sure to get into the habit of performing this
operation.
3
Bring the filter cube into the optical path.
Bring the desired cube into the optical path by turning the
filter cube switching knob.
(Confirm the address of the filter cube to be used, and
match the switching knob with the number.)
Selecting a filter cube
A filter cube consists of three types of optical
components: an excitation filter (EX filter), a
barrier filter (BA filter), and a dichroic mirror (DM).
Select the filter cube with the appropriate
combination of optical components for the
characteristics of the specimen and the
fluorescence dye.
4
Fully open the field diaphragm of the epi-fluorescence attachment.
Push in the field diaphragm lever of the epi-fluorescence
attachment to open the diaphragm fully.
A1
-
2
-
3
-
4
B1
-
2 / 3
-
4
C1
-
2
-
3
-
4
1
1
4
2 3 4
CUBE
Fully opening the field diaphragm
Opening the shutter
5
Turn on the mercury lamp.
See your illuminator’s manual for details.
6
Open the shutter.
Set the shutter open/close lever of the epi-fluorescence
attachment to position “O” to open the shutter.
Light shielding plate of the
epi-fluorescence attachment
The light shielding plate protects the observer’s
eyes from reflected ultraviolet light, which is
originally emitted from the objective at the
specimen.
Filter cube
switching knob
Filter cube
nameplate window
Shutter opened (O)
position
Field diaphragm
leve
r
Ligh
t
shielding
plate
Shutter closed (C)
position