Chapter 1 Microscopy Procedures
19
Microscopy Procedures
Simple Polarizing Microscopy
Chapter 1-3
13
Adjust the orientation of the analyzer and the
polarizer.
(1) Fully open the aperture diaphragm.
(2) Pull out one eyepiece from the tube.
(3) Look into the eyepiece sleeve and rotate the whole polarizer unit until you can identify a dark cross.
(You will see black stripes that change shape as you rotate the polarizer unit.)
(4) Tighten the fixing screw of the polarizer unit to fix the
polarizer.
(5) Place the eyepiece back into the tube.
Dark cross (crossed Nicols)
You will see a dark cross when the orientation o
the analyzer orthogonally crosses that of the
polarizer.
Dark cross
14
Bring an arbitrary objective into the optical path.
Turn the nosepiece to bring the desired objective into the optical path.
Swing-out condenser 1-100x
Mounting a 1-100x swing-out condenser on the
Ci-L main body when using the 1x objective may
result in uneven illumination around the field o
view.
15
Focus on the specimen.
(1) Look into the eyepiece, and adjust the brightness of the field of view by turning the dia-illumination brightness
control knob. You can also adjust the brightness with an ND filter for Ci-S.
(2) Rotate the stage knob to move the stage and bring the target into the optical path.
(3) If the specimen is not in focus, turn the focus knob to focus on it.
16
Adjust the field diaphragm.
Turn the field diaphragm dial to adjust the field diaphragm
so that it almost circumscribes the field of view.
Size of the field diaphragm
Normally, adjust the field diaphragm so that it
almost circumscribes the field of view. Opening
the field diaphragm too much results in stray light
entering the field of view, generating flare and
reducing the image contrast. In addition, the
sample will become decolorized over a wide
area.
Field diaphragm's adjustment timing
Be sure to adjust the field diaphragm each time
you change the objective.
Circumscribe around the field of view
Adjusting the field diaphragm
Field of viewField Diaphragm
Field
diaphragm
dial
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