Chapter 1 Microscopy Procedures
11
Chapter 1-2
Microscopy Procedures
Phase Contrast Microscopy
4
Move the condenser turret to the [A: empty]
position.
Turn the condenser turret until the [A: empty] symbol
comes to the front, and align the hole with the optical path.
Aperture diaphragm of the condenser
Positioning the condenser turret at positions othe
than [A: empty] deviates the aperture diaphragm
from the optical path.
CLAMP
TORQUE
PHASE CONTRAST
0.90 DRY
JAPAN
A
Move the condenser turret to the [A: empty]
position.
5
Bring the 10x Ph objective (Ph1) into the optical
path.
6
Place a specimen on the stage, and move the
stage to bring the target into view.
7
Focus on the specimen. (→See Chapter 2 “2 Focusing on the Specimen” for details)
8
Adjust the diopter. (→See Chapter 2, “4 Adjusting the Diopter” for details)
9
Adjust the interpupillary distance.
10
Focus and center the condenser. (See Chapter 2, “5 Focusing and Centering the Condenser” for details)
A: empty
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