Chapter 2 Troubleshooting
31
Troubleshooting
1.3
Differential Interference Contrast Microscopy
Problem Cause Measure
The universal condenser's turret is at the
intermediate position.
Rotate it to the click stop position.
(→See Chapter 3 “7.2 Using the NI-CUD Universal
Condenser (Dry)” in the “Operation”.)
The DIC slider is at the intermediate position for
an oil condenser.
Push in the slider until it reaches the limit.
(→See Chapter 3 “7.3 Using the D-CUO DIC Condenser
(Oil)” in the “Operation”.)
The DIC module on the objective is at the
intermediate position.
Attach it correctly.
(→See Chapter 3 “15.2 Using Optical Elements” in the
“Operation”.)
Incomplete attachment of nosepiece.
Attach it correctly.
(→See Chapter 1, “3 Assembly Method, 14 Attach the
nosepiece” in the “Assembly/Maintenance”.)
The polarizer and an analyzer are at the
intermediate position.
Switch over correctly.
(→See Chapter 3 “15.2 Using Optical Elements” in the
“Operation”.)
Lack of visibility around
periphery of field of view.
The lambda plate is at the intermediate position.
Make sure that it is attached to the limit.
(→See Chapter 3 “15.2 Using Optical Elements” in the
“Operation”.)
The polarizer is out of the optical path.
Bring the polarizer into the optical path.
(→See Chapter 2 “4 Differential Interference Contrast
Microscopy - 14 Adjust the orientation (vibration direction)
of the polarizer and analyzer” in the “Operation”.)
The analyzer is out of the optical path.
Bring the analyzer into the optical path.
(→See Chapter 2 “4 Differential Interference Contrast
Microscopy - 14 Adjust the orientation (vibration direction)
of the polarizer and analyzer” in the “Operation”.)
A correct DIC module on the condenser has not
been selected.
Select the DIC module suitable for the objective
used.
(→See Chapter 1 “3 Assembly Method - 13 Attach the
condenser, ■ Attaching the optical module to the universal
condenser”, and “■Attaching the DIC module to DIC
condenser oil” in the “Assembly/Maintenance”.)
The DIC slider on the objective is out of the
optical path.
Bring the DIC slider on the objective into the optical
path.
(→See Chapter 2 “4 Differential Interference Contrast
Microscopy - 16 Attach the DIC slider (on the objective) to
the nosepiece” in the “Operation”.)
Incorrect combination of the objective and the
DIC slider on the objective.
Use the DIC slider suitable for the objective.
(→See Chapter 1 “3 Assembly Method - 13 Attach the
condenser, ■ Attaching the optical module to the universal
condenser”, and “■Attaching the DIC module to DIC
condenser oil” in the “Assembly/Maintenance”.)
No contrast.
The immersion oil is not sufficient in the oil
condenser, and air bubbles are between the
slide and the condenser.
Apply the immersion oil again.
(→See Chapter 3 “12 Oil Immersion/Water Immersion” in
the “Operation”.)
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