Chapter 2 Microscopy Techniques
66
6 Overwrap the annular diaphragm image with the
annular image of the phase plate using the
centering telescope.
(1) Remove one eyepiece, attach the centering
telescope, and focus on the annular diaphragm
image.
Focus on the specimen by turning the eyepiece while
pressing down on the flange ring of the centering
telescope.
(2) Overwrap the annular diaphragm image with the
annular image of the phase plate.
Insert the tool from the hole on the front of the
condenser, turn the annular diaphragm centering
screw of the phase contrast slider, and overlap the
annular image of the phase plate of the objective with
the annular diaphragm image.
Centering adjustment of the annular diaphragm
(3) Replace the centering telescope with the
eyepiece.
(1) Focus on the annular diaphragm image using the
centering telescope.
(2) Adjust the position of the condenser so that the annula
image of the phase plate and the annular diaphragm
image overlap.
7 Adjust the aperture diaphragm and field
diaphragm.
(1) Set the aperture diaphragm lever of the
condenser at the fully open position (leftmost).
(2) Turn the field diaphragm dial to resize the field
diaphragm image so that it is circumscribed with
the field of view.
Adjust the field diaphragm and the aperture
diaphragm.
8 Attach the GIF filter (green interference filter) to
the field lens.
The contrast of the Ph image is improved by using
the GIF filter.
Eyepiece of the
centering telescope
Flange
Phase ring
Annula
diaphragm
perture diaphragm lever
• At the fully open
position
Field diaphragm dial
• Adjust it so that the
image is circumscribed
with the field of view
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