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Nikon ECLIPSE Si - Page 69

Nikon ECLIPSE Si
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Chapter 2 Microscopy Techniques
67
9 Observe the specimen using the phase contrast
microscopy method.
Turn the stage and focus knobs to observe the
specimen.
To observe the specimen with a different
magnification, repeat steps 3 to 7.
10 Switch to bright-field microscopy.
(1) Remove the phase contrast slider to remove the
annular diaphragm from the optical path.
Remove the slider knob on the left side of the
condenser, and pull the slider out from the right side
of the condenser.
(2) Bring the objective for bright-field microscopy
into the optical path.

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