Why is there no phase contrast effect on my Nikon Microscope?
- MMichael MullenSep 3, 2025
If you're not getting a phase contrast effect on your Nikon Microscope, ensure you're using a Ph objective. Also, check that the condenser annular diaphragm is in the optical path and matches the Ph code of the objective, and that it is centered. If using a system condenser, make sure the aperture diaphragm is fully open.