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Nikon Microphot-FXA User Manual

Nikon Microphot-FXA
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Table of Contents

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Nikon Microphot-FXA Specifications

General IconGeneral
BrandNikon
ModelMicrophot-FXA
CategoryMicroscope
LanguageEnglish

Summary

Handling Precautions

Handle Carefully

Gentle handling to avoid sharp impacts.

Carrying the Microscope

Remove lamphouse, grip indicated handholds.

Microscope Location

Select location with limited exposure to dust, vibration, etc.

Line Voltage and Fuse

Confirm voltage selection and correct fuse rating.

Lamphouse

Keep away from volatile substances due to heat.

High-Sensitivity Photomultiplier

Avoid overexposure warning or strong light exposure.

Film Sensitivity Settings

Set film sensitivity carefully, e.g., ISO 6 or lower.

Care and Maintenance

Lens Cleaning

Use soft brush or gauze; moisten with alcohol for persistent dirt.

Cleaning Painted or Plastic Surfaces

Use mild soap/water; avoid organic solvents; handle lettering gently.

Never Dismantle

Avoid dismantling to prevent impaired efficiency and accuracy.

When Not in Use

Cover instrument and store in a place free from moisture and fungus.

I. NOMENCLATURE

System Components

Identifies various parts of the microscope system with labels.

Rear

Details connectors and mounts on the rear of the microscope.

Upper Right Side

Describes camera mounts, TV mount, and controls on the upper right.

Upper Left Side

Details camera mounts, magnifier, ND filter, and other controls on the upper left.

Lower Right Side

Explains controls like motorized nosepiece, reticle illumination, and filter cassette.

Lower Left Side

Details stage controls, condenser, LCD, and focus knobs on the lower left.

Dark Box (FX-35DX)

Explains film loading, advance, rewind, and DX cable connection.

Large Format Adapter

Describes mounting the large format adapter and film holders.

Power Supply Unit

Details the front and rear panels of the power supply unit.

Slide-Out Keyboard

Explains the LCD display, numerical keys, and function keys.

LCD Display

Details the main screen components, camera displays, and mode indicators.

II. BASIC MICROSCOPY

Turn on the power

Steps to power on the microscope system.

Check the illumination optical path

Verifying the diascopic illumination indicator.

Set the lamp voltage to 7.0

Adjusting lamp voltage using the brightness control switch.

Select the ND32 and NCB11 filters

Inserting specific filters into the optical path.

Move the 10× objective into the optical path

Rotating the nosepiece to select the 10x objective.

Place the specimen on the stage

Instructions for correctly placing the specimen.

Raise the condenser

Raising the condenser to its full limit.

Fully open the field aperture and condenser aperture diaphragm

Opening the field and condenser aperture diaphragms.

Raise the stage

Raising the stage and centering the condenser.

Position the specimen

Moving the stage to position the specimen in the viewfield.

Push in the OB/PHOTO light path selector knob

Engaging the photomask in the optical path.

Focus

Using the triaxial focus knob to focus the specimen.

Adjust the eyepiece diopter

Correcting focus for observer's eyesight using diopter adjustment.

Adjust the interpupillary distance

Adjusting eyepiece tubes for a single, clear viewfield.

Center the condenser

Centering the condenser using the field diaphragm image.

Switch to the 40 × objective and re-center the condenser

Switching to the 40x objective and re-centering the condenser.

Switch to the desired objective

Selecting the objective and adjusting the field diaphragm.

Adjust the brightness

Adjusting brightness using ND filters or the control switch.

Adjust the condenser diaphragm

Setting the condenser diaphragm to 70-80% of the objective's N.A.

III. CAMERA MOUNTING AND FILM LOADING

System Composition

Overview of cameras that can be mounted on the MICROPHOT-FXA.

35mm Dark Box (FX-35DX/FX-35W)

Procedures for loading film and attaching the 35mm dark box.

Large Format Camera System

Instructions for mounting large format adapters and film holders.

Nikon F3

Guidance on using the Nikon F3 camera body with the microscope.

IV. PHOTOMICROGRAPHIC PROCEDURE

Set AUTO, AVE, ↑, and 9.0

Initial settings for AUTO mode: measurement range, illumination, lamp voltage.

Select Camera

Setting the optical path for the camera to be used.

Select Optical Path

Pushing selector knobs to engage the photomask.

Compose the Frame

Positioning the specimen and adjusting focus using stage and photomask.

Check Exposure Time

Adjusting exposure time, lamp voltage, and measurement area.

Depress Shutter Key

Activating the shutter and interrupting exposure.

Advance the Film One Frame

Advancing the film to the next frame or changing film.

V. COMPONENT OPERATION

Changing Illumination Optical Paths

Switching between Diascopic and Episcopic illumination methods.

Fixing the Optimum Voltage for Photomicrography

Enabling Voltage Lock for stable lamp voltage at 9V for optimal color balance.

Improving Color Tone

Adjusting lamp voltage and using color compensation filters for optimal color reproduction.

Easier Operation (Using the control grip)

Performing shutter, nosepiece rotation, and reticle illumination with control grips.

Accurate Focusing

Using the triaxial focus knob for precise specimen focusing.

View Adjustment (Condenser aperture diaphragm operation)

Adjusting aperture diaphragm for optimal contrast, resolution, and depth of focus.

Eliminating Flares (Field aperture operation)

Adjusting the field diaphragm to prevent stray light and improve contrast.

Optimal Photo Composition (Stage rotation)

Loosening stage clamp to rotate stage for convenient photo composition.

Setting Exposure Time (Photomicrographic modes)

Operating under different photomicrographic modes: AUTO, MANUAL, TIME, etc.

Increasing Magnification

Using the intermediate magnification selector for various magnifications.

Selecting Magnification Displays

Choosing between objective, film, or observation surface magnification displays.

VI. TROUBLESHOOTING TABLES

Optical

Troubleshooting optical issues like vignetting, dirt, and poor image quality.

Operational

Troubleshooting operational issues like focus, specimen movement, and eye fatigue.

Electrical

Troubleshooting electrical issues like lamp failure, insufficient brightness, and flickering.

Photomicrographic

Troubleshooting photomicrographic issues like sharpness, image fog, brightness, and contrast.

Error Message

Explains error messages and their corresponding causes and countermeasures.

ELECTRICAL SPECIFICATIONS

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