Olympus bx40 - Page 23

BX40
Use
of
Immersion
Objectives
(Fig.
59)
.
Focus
on
the
specimen
with
a
low
power
objective.
Place
a
drop
of
immersion
oil
(provided)
onto
the
specimen
at
the
portion
to
be
observed.
.
Turn
the
revolving
nosepiece
to
move
the
oil
immersion
lens
into
the
light
path,
then
focus
using
the
fine
adjustment
knob.
If
the
condenser
marking
shows
а
numerical
aperture
(NA)
of
1.0
or
more,
the
number
applies
only
when
oil
is
present
between
the
slide
glass
and
the
top
element
of
the
condenser.
When
oil
is
not
present,
the
NA
is
about
0,9,
*
Since
any
bubbles
in
the
oil
will
impair
the
image,
make
sure
that
the
oil
is
free
of
bubbles.
a.
To
check
for
bubbles,
remove
the
eyepiece
and
fully
open
field
and
aperture
iris
diaphragm,
then
look
at
the
exit
pupil
of
the
objective
inside
the
observation
tube.
(It
should
appear
round
and
bright.)
b.
To
remove
bubbles,
rock
the
nosepiece
slightly
to
move
the
oil
immersion
objective
back
and
forth
a
few
times.
4.
After
use,
remove
oil
from
the
objective
front
lens
by
wiping
it
carefully
with
gauze
dampened
with
a
very
small
quantity
of
7
parts
ether:
3
parts
alcohol
solution,
or
with
Xylol.
*
Using
too
much
Xylol
can
dissolve
the
lens
adhesive.
*
Use
a
trinocular
observation
tube
(U-TR30)
for
taking
photomicrographs.
Photomicrograph
can
be
performed
using
either
the
PM-10,
the
PM-20,
or
the
PM-30
photomicrographic
system.
Procedures
for
using
the
photomicrographic
unit
are
described
in
respective
instruction
manual.
Procedures
specific
to
this
microscope
are
given
below.
a Single Port Tube Attachment (U-SPT) (Fia. 60)
1. Using the Allen wrench, loosen the clamping screw ©) on the trinocular
lens photo tube.
2. Align the vertical index line @ with the index dot @ on the single port
tube, then insert the single port tube into the photo tube.
3. Securely tighten the clamping screw ©.
QINHINNI
3Η!
ΩΝΙςΩ
FA