14
CX31
4-6 Immersion Objectives
Fig. 24
Using the Immersion Objectives (Fig. 24)
1
#Be sure to use the provided Olympus immersion oil.
When the oil of other make is used the surface of the Top lens of
Condenser may be damaged.
1. Focus on the specimen by switching the objectives fro the lowest
power to highest power.
2. Before engaging the immersion objective in the light path, place a
drop of immersion oil provided with the 100X objective combination
model onto the specimen at the area to be observed.
3. Turn the revolving nosepiece to engage the immersion objective, then
focus using the fine adjustment knob.
#Since air bubbles in the oil will affect the image quality, make sure
that the oil is free of bubbles.
To remove bubbles, turn the revolving nosepiece to move the oil
immersion objective back and forth a few times.
}If the condenser engraving shows a numerical aperture (NA) of 1.0 or
higher, the number applies only when oil is applied between the slide
glass and the top surface of the condenser. When oil is not present,
the NA is about 0.9.
4. After use, remove oil from the objective front lens by wiping with gauze
slightly moistened with an ether (70%)/alcohol (30%) mixture.
Caution in use of the immersion oil
If immersion oil penetrates in your eye or attaches to your skin,
apply the following treatment immediately.
Eye: Rinse with fresh water (for more than 15 minutes)
Skin: Wash with water and soap.
When the appearance of your eye or skin is altered or pain
continues, immediately consult the doctor.
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