1) Excessive image contrast.
Condenser is stopped in high posi-
Lower condenser.
tion.
Aperture diaphragm is stopped Open diaphragm.
down excessively.
?) Resolution problems: Objective is not correctly posi- Click nosepiece into place.
l
Image is not sharp. tioned in light path.
0 Insufficient contrast.
l
Image details lack definition.
Aperture diaphragm is stopped
Adjust aperture iris opening pro-
down excessively or opened too perly.
much.
Correction collar is not adjusted
Looking at specimen image, rotate
correctly.
correction collar until optimum
focusing position can be found.
Dust on condenser, objective, eye- Clean.
piece, culture vessel, etc.
Thickness of vessel bottom is more
Use a vessel of bottom thickness
than 2 mm.
less than 2 mm.
‘) No effective phase contrast is Bright field objective is used.
Use phase objective.
obtained.
Light annulus is not matched with Match light annulus to objective.
objective.
Light annulus and phase annulus Center them correctly.
are not centered.
g) Specimen image is partially out Objective is not correctly in light Slightly rotate nosepiece until it
of focus. path. clicks into place.
Specimen is not correctly place on
Place specimen correctly.
stage.
Vessel bottom is not flat. Use an evenly-flat bottomed vessel.
h) Image is blurred. Condenser is not correctly centered.
Center condenser.
Light source is not correctly cen-
Center light source correctly,
tered.
Condenser holder is tilted up. Lower it to stop position.
2. Electrical adjustment
a) Light source is too bright even at
Minimum line voltage adjustment
Adjust the knob until bulb is dimly
lowest bulb voltage. knob is not correctly adjusted.
lit with sliding control lever at
lowest position (nearest to you).
b) Light flickers and intensity is un-
Line voltage is unstable.
Use voltage stabilizer.
stable.
Bulb filament is likely to burn out. , Replace bulb.
Loose electrical connection.
I
i Tighten connections.
c) Fuse burns out too often.
Fuse is not a standard one. 1 Use standard fuse.
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