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Olympus IX71 User Manual

Olympus IX71
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INSTRUCTIONS
IX71/IX51
INVERTED RESEARCH MICROSCOPE/
INVERTED BASIC MICROSCOPE
A X 7 3 1 9
This instruction manual is for the Olympus Inverted Microscopes Models IX71 and IX51. To
ensure the safety, obtain optimum performance and to familiarize yourself fully with the use
of the microscope, we recommend that you study this manual thoroughly before operating
the microscope. Retain this instruction manual in an easily accessible place near the work
desk for future reference.

Table of Contents

Other manuals for Olympus IX71

Questions and Answers:

Olympus IX71 Specifications

General IconGeneral
TypeInverted Research Microscope
Optical SystemUIS2 (Universal Infinity System)
ObjectivesUIS2 series
Nosepiece6-position
Illumination12V 100W halogen
FocusingCoarse and fine focusing
Observation MethodsBrightfield, Phase contrast, DIC, Fluorescence
Objective LensUIS2 Objectives
Camera PortTrinocular port

Summary

MODULE NOMENCLATURE

Unpacking Precautions

Safety precautions to follow when unpacking the microscope.

SAFETY PRECAUTIONS

Safety Symbols Explained

Explanation of symbols used in the manual for safe operation.

General Warnings and Precautions

General warnings regarding handling and usage precautions.

Getting Ready

Initial steps and precautions before operating the microscope.

Maintenance and Storage

Cautionary Statements

General cautionary statements regarding improper use of the equipment.

Intended Use Guidelines

Specifies the designed purpose and application of the instrument.

CONTROLS

Microscope Controls Overview

Identifies various controls and their locations on the microscope.

USING THE CONTROLS

Power Supply and Microscope Frame Setup

Explains the setup and operation of power supply units and the microscope frame.

Light Path Selection

Describes how to switch between different light paths for observation.

Magnification Adjustment (IX71)

Details how to change the magnification on the IX71 model.

Frame Clamping Plate Usage

Information on using modules for clamping the microscope frame onto anti-vibration platforms.

IX71 Option Button Function

Explains the function of the option button specific to the IX71 model.

Focusing Block Operations

Adjustment Knob Rotation Direction

Explains the directional control of coarse and fine adjustment knobs.

Coarse Adjustment Knob Tension

Describes how to adjust the tension of the coarse adjustment knob.

Fine Adjustment Knob Detachment

Instructions on how to detach the fine adjustment knob for better manipulation.

Pre-focusing Lever Operation

Explains the function and use of the pre-focusing lever for easier focusing.

Stage Operations

Specimen Placement on Stage

Instructions on how to place specimens on various stage types.

Specimen Movement Controls

Describes methods for moving the specimen using stage controls.

Grounding Wire Connection

Instructions for connecting a grounding wire for electrophysiological experiments.

Stage Knob Tension Adjustment

How to adjust the rotation tension of stage control knobs.

Observation Tube Adjustments

Interpupillary Distance Adjustment

Steps to adjust the distance between eyepieces for comfortable viewing.

Diopter Adjustment Procedure

Guide to adjusting the diopter for clear focus for each eye.

Eye Shade Usage

Instructions on using eye shades for better viewing comfort.

Illumination Column IX2-ILL100

Tilting the Illumination Column

Procedure for tilting the illumination column to create working space.

Filter Mounting Procedure

Instructions on how to mount filters into the filter holder.

Field Iris Diaphragm Adjustment

How to adjust the field iris diaphragm for optimal illumination.

Condenser Height Knob Tension

Adjusting the tension of the condenser height adjustment knob.

Manipulator Mounting Steps

Steps for mounting a manipulator.

Illumination Column IX2-ILL30

Aperture Iris Diaphragm Usage

How to adjust the aperture iris diaphragm for image quality.

Condenser Lens Removal

Procedure for removing the condenser lens to increase working distance.

Filter Mounting Procedure

Instructions on how to mount filters into the filter holder.

Filter Usage Guide

Guide to using various filters for effective observation and photography.

Condenser Operations

Centering the Condenser

Step-by-step guide to centering the condenser for optimal illumination.

Aperture Iris Diaphragm Adjustment

Explains the function and adjustment of the aperture iris diaphragm.

Condenser Holder Flipping

Procedure to flip up the condenser holder for easier access.

Objective Handling

Oil-Proof Cap for UIS Series

How to mount oil-proof caps on UIS series objectives for protection.

Correction Collar Adjustment

How to adjust the correction collar based on vessel bottom thickness.

Immersion Objective Usage

Guidelines for using oil immersion objectives, including handling oil.

OTHER OBSERVATION METHODS

Phase Contrast Observation (IX2-ILL100)

Detailed procedure for phase contrast observation using specific illumination columns.

Phase Contrast Optical Elements

Lists optical elements and objectives for phase contrast.

Attaching Phase Contrast Elements

Steps to attach phase contrast optical elements.

Centering Phase Contrast Ring Slit

Procedure for centering the phase contrast ring slit for optimal performance.

Phase Contrast Observation (IX2-ILL30)

Phase Contrast Optical Elements

Lists optical elements and objectives for phase contrast with IX2-ILL30.

Attaching Optical Elements

Instructions for attaching optical elements for phase contrast.

Attaching Phase Contrast Slider

Steps to attach the phase contrast slider.

Centering Phase Contrast Ring Slit

Procedure for centering the phase contrast ring slit.

Differential Interference Contrast (IX2-ILL100)

DIC Optical Elements and Sliders

Lists DIC optical elements, objectives, and sliders.

Attaching DIC Optical Elements

Steps to attach DIC optical elements.

Attaching Analyzer and DIC Slider

How to attach the analyzer and DIC slider.

PHOTOMICROGRAPHY AND TV OBSERVATION

Photomicrography Procedures

Procedures for taking photographs using the microscope.

Straight Photo Tube Attachment

Steps for attaching the straight photo tube for photomicrography.

Photo Eyepiece Attachment

How to attach a photo eyepiece for photomicrography.

Photomicrographic System Connection

Instructions for connecting the photomicrographic system.

Photomicrography Light Path Switching

How to switch the light path for photomicrography.

Focus Adjustment for Photography

Steps for adjusting focus for photomicrography.

Illumination Adjustment for Photography

Proper adjustment of illumination for optimal photographic results.

Illumination Heat Effects

Information on heat generated by illumination and its effects.

Filter and Lamp Intensity Settings

Guidance on selecting filters and lamp intensity for photography.

TV Observation Setup

Camera Attachment to Side Port

How to attach a video camera to the microscope's side port.

Camera Attachment to Trinocular Tube

Instructions for attaching a camera to the trinocular observation tube.

Camera Attachment to Monocular Tube

How to attach a camera to the monocular tube.

Camera Attachment to Back Port

Information on attaching a camera to the lower back port.

Camera Attachment to Bottom TV Port

How to attach a camera to the bottom TV port.

General TV Observation Procedures

General procedures for TV observation, including adjustments.

TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION PROCEDURE

Observation Procedure Steps

Flowchart detailing the steps for basic brightfield observation.

Controls Used for Observation

Lists the specific controls and their page references for the observation procedure.

TROUBLESHOOTING GUIDE

Optical System Issues

Troubleshooting issues related to the microscope's optical system.

SPECIFICATIONS

Module Specifications Overview

Technical specifications for various microscope modules and components.

ASSEMBLY GUIDE

Assembly Diagram Overview

Visual overview of the module assembly sequence.

Detailed Assembly Procedures

Step-by-step procedures for assembling microscope components and modules.

POWER SUPPLY CORD SELECTION

Power Cord Specifications

Details on voltage, current, and temperature ratings for power cords.

LAMP HOUSING INSPECTION

Inspection Checklist

A checklist for inspecting the lamp housing for maintenance and safety.

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