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Olympus U-LH100HG - User Manual

Olympus U-LH100HG
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INSTRUCTIONS
A X 7 6 2 5
BX-URA2
BX-RFA
U-LH100HGAPO
U-LH100HG
Power Supply Unit
U-25ND6-2
U-25ND25-2
U-25ND50-2
U-RSL6
U-RSL6EM
BX-RFSS
U-EXBABG
U-EXBAUB
U-EXBAUG
REFLECTED
FLUORESCENCE
SYSTEM
This instruction manual is for the Olympus Reflected Fluorescence System. To ensure the safety,
obtain optimum performance and to familiarize yourself fully with the use of this system, we recom-
mend that you study this manual thoroughly before operating the microscope. Retain this instruc-
tion manual in an easily accessible place near the work desk for future reference.
This publication is printed on 100% recycled paper

Table of Contents

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Questions and Answers:

Olympus U-LH100HG Specifications

General IconGeneral
BrandOlympus
ModelU-LH100HG
CategoryMicroscope
LanguageEnglish

Summary

IMPORTANT

SAFETY PRECAUTIONS

Safety guidelines for handling the precision instrument system.

Getting Ready

General Precautions for Use

Guidelines for safe and proper operation of the reflected fluorescence system.

Maintenance and Storage

Cleaning and Storage Procedures

Instructions for cleaning components and proper storage of the equipment.

Caution

Usage Cautions

Warnings about improper usage that may lead to injury or damage.

I. REFLECTED FLUORESCENCE OBSERVATION

1 NOMENCLATURE

Identification and diagram of the reflected fluorescence system components.

2 REFLECTED FLUORESCENCE OBSERVATION PROCEDURE

Step-by-step guide for performing reflected fluorescence observations.

3 USING THE CONTROLS

Detailed instructions for operating the various controls of the system.

4 SIMULTANEOUS FLUORESCENCE OBSERVATIONS

Methods for combining reflected fluorescence with other observation techniques.

5 TROUBLESHOOTING GUIDE

Common problems and their solutions for the fluorescence system.

6 SPECTRAL CHARACTERISTICS OF FILTERS

Graphs showing the spectral transmission of various filters used in the system.

7 SPECIFICATIONS

Technical specifications for the vertical illuminators and mercury lamp housing.

8 OPTIONAL MODULES

1 6-Position Filter Slider U-RSL6

Details on mounting and using the 6-position filter slider for excitation and ND filters.

2 6-Position Barrier Filter Slider U-RSL6 EM

Instructions for mounting and using the 6-position barrier filter slider.

3 Rectangle Field Stop BX-RFSS (for exclusive use with the BX-RFA)

Description and installation of the rectangular field stop accessory.

4 Exciter Balancers U-EXBABG;EXBAUB;EXBAUG (for exclusive use with the BX-RFA)

How to use exciter balancers for adjusting excitation light intensity of fluorochromes.

9 ASSEMBLY

9-1 Assembly Diagram

Diagram illustrating the sequence of assembling microscope modules.

9-2 Detailed Assembly Procedures

Step-by-step instructions for attaching fluorescence mirror units.

II. REFLECTED OBSERVATIONS (BX-URA2 Only)

1 CONFIGURATION OF REFLECTED OBSERVATION SYSTEM

Overview of components for reflected observation systems using the BX-URA2.

2 ASSEMBLY

Assembly procedures specific to certain items not covered with fluorescence modules.

3 FIELD IRIS AND APERTURE IRIS DIAPHRAGM ADJUSTMENTS

Procedures for adjusting field and aperture iris diaphragms for reflected observation.

4 OBSERVATIONS

Procedures for various types of reflected light observations.

4-1 Reflected Light Brightfield;Darkfield Observations

How to set up and perform brightfield and darkfield observations.

4-2 Reflected Light Nomarski Differential Interference Contrast (DIC) Observation

Steps for performing Nomarski DIC observation under reflected light.

4-3 Reflected Light Simple Polarized Light Observation

Procedure for simple polarized light observation using the reflected illuminator.

5 OPTICAL CHARACTERISTICS UIS2 (UIS) Series for Reflected Light Observation

Objective Naming Convention

Explanation of how objective names are structured and what they signify.

Glossary of Optical Terms

Definitions of terms used in optical characteristics tables.

6 TROUBLESHOOTING GUIDE

Reflected Light Observation Modes

Troubleshooting common issues in reflected light observation modes.

PROPER SELECTION OF THE POWER SUPPLY CORD

Specifications

Details on voltage, current, and fittings for power supply cords.

Certified Cord Table

List of agencies and certification marks for approved power supply cords.

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