www.pall.com/biotech 13
Table 2
Temperature correction factors (TCF
20 °C
) for Cadence ILC module permeability
T °C TCF
20 °C
T °C TCF
20 °C
4 1.57 28 0.83
5 1.52 29 0.81
6 1.47 30 0.80
7 1.43 31 0.78
8 1.39 32 0.76
9 1.35 33 0.75
10 1.31 34 0.73
11 1.27 35 0.72
12 1.24 36 0.70
13 1.20 37 0.69
14 1.17 38 0.68
15 1.14 39 0.66
16 1.11 40 0.65
17 1.08 41 0.64
18 1.05 42 0.63
19 1.03 43 0.62
20 1.00 44 0.61
21 0.98 45 0.60
22 0.95 46 0.59
23 0.93 47 0.58
24 0.91 48 0.57
25 0.89 49 0.56
26 0.87 50 0.55
27 0.85
u
Procedure F — Determine the Hold-Up Volume of the Cadence Setup and Module
This procedure includes steps to determine hold-up volume of the combined feed to retentate
flow path.
HELPFUL INFORMATION!
This step should be performed prior to using the system for
processing for the first time. This step is not required for future process runs utilizing the
same system/plumbing and same module size.
Follow these steps to determine the hold-up volume:
1. Open the feed and retentate lines and close both permeate lines.
2. Direct the retentate line to a collection vessel.
3. Disconnect the feed line from previous supply of fluid. Using the system pump to supply
clean air to the feed port, pressurize the feed to 0.7 barg (10 psig). Alternatively, another
source of oil-free sterile filtered compressed air can be used to pressurize the feed to
0.7 barg (10 psig).
4. Wait until all fluid in the module is pushed out of the retentate port. Only air should be
flowing from the retentate port.
5. Stop the air flow into the feed port.
6. Measure the retentate volume and record the value as the system hold-up volume.
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