www.pall.com/biotech 7
Figure 2
Cadence Inline Concentrator T12 module port locations
Permeate 2 port
Permeate 1 port
Retentate port
Feed port
3. Application of Single-Pass Tangential Flow Filtration
Single-pass tangential flow filtration is a patented novel technology that enables the concentration
of process solutions with the simplicity of direct flow filtration, and can be used for:
• Continuous product concentration
• In-process volume reduction
• In-process salt reduction
• Processing of fragile biomolecules
Depending upon the format size of the Cadence ILC module, product volumes of several hundred
liters can be processed. The modules can be coupled (before or after) to other process steps
(e.g., chromatography).
3.1 Process Variables
Optimum processing conditions and sizing for the Cadence ILC module may need to be
determined via trials, considering the following processing variables:
• Product characteristics (i.e., concentration, viscosity, temperature, and shear sensitivity)
• Concentration factor ranges
• Feed pressure or feed flow rate
Process control with the Cadence ILC module can be easily achieved by setting either the
feed pressure or feed flow rate to reach processing targets. Please contact Pall for single-use
Allegro™ systems or assemblies to operate the Cadence ILC module.
3.2 Proper Use
The following chapters describe the procedures to properly install and prepare Cadence ILC
modules. You may need to modify some aspects of the procedures to fit your specific
objectives.
4. An Overview of Cadence ILC Processing
4.1 The Four Parts of a Single-Pass TFF Process
The SPTFF process can be divided into four parts:
• Part 1 — The first part involves connecting the Cadence ILC module and preparing it for use.
This part (called pre-use conditioning) involves several sequential steps.
• Part 2 — The second part (called processing) involves using the Cadence ILC module to
meet the filtration objectives. Processing includes product filtration and product recovery.
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