Using the Spectrometer . 61
Cell Type Cell
Internal
Width
Pathlength Minimum
Volume
Required
Part
Number
Height of
liquid slightly
more than
height of
beam
2 mm 1 cm 150 μL B0079404
(pair)
4 mm 1 cm 300 μL B0079402
(pair)
Cell
Volume
Pathlength Minimum
Volume
Required
Part
Number
Cell window
completely
filled with
liquid
0.5 μL 0.01 cm 2 μL B0510076
2.5 μL 0.5 cm 5 μL B0510077
5 μL 0.1 cm 10 μL B0510078
5 μL 1.0 cm 10 μL B0505823
30 μL 1.0 cm 50 μL B0190608
NOTE: You should align microcells very carefully in the radiation beam by following the
procedures in
Aligning the Single-Cell Holder
on page 58. When aligning microcells,
fill each cell with at least the minimum volume of liquid specified in the above table to
make sure that the liquid meniscus is not in the radiation beam.