60 . High-Performance Lambda Spectrometers Hardware Guide
Fine Alignment
If fine alignment is necessary, proceed as follows:
1. Slew the monochromator to your measurement wavelength or to 460 nm.
2. Call up a method that uses transmission (%T) as the ordinate.
OR
If necessary, change the ordinate mode to transmission.
3. Remove the reference cell from the sample compartment.
4. Make horizontal fine alignment adjustments to the sample cell holder (locking screws
and milled posts loosened) to obtain the highest possible transmittance reading on the
display.
Close the sample compartment cover while measuring transmittance.
5. Make fine alignment adjustments using the vertical adjustment screw, again to obtain
the highest possible reading.
Close the sample compartment cover while measuring transmittance.
6. When you are satisfied with the alignment, tighten the milled posts and the locking
screws on the cell holder.
7. Reinstall the reference cell in the reference cell holder.
The sample cell remains in its holder.
8. Repeat steps 4 to 6 with the reference cell holder, but this time obtain the lowest
possible transmittance reading on the display.
This completes the fine alignment procedure.
NOTE: When the cell holder has been aligned once, you can take it out and reinstall it
without aligning it again.
Minimum Volume Applications
To measure minimum sample volumes, use microcells (offered by PerkinElmer).
The minimum sample volume required is a function of the internal width, or volume, of the
cell and is specified in the following table.
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