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PerkinElmer LS 55 User Manual

PerkinElmer LS 55
202 pages
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LS 55
User’s Guide

Table of Contents

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PerkinElmer LS 55 Specifications

General IconGeneral
TypeLuminescence Spectrometer
DetectorPhotomultiplier Tube (PMT)
Wavelength Range200-900 nm
Light SourceXenon Lamp
Data Sampling Interval0.1-5 nm
Data OutputRS-232
Power Requirements100-240 VAC, 50/60 Hz

Summary

Warnings and Safety Information

Safety Information

Details safety requirements for the LS 55 Luminescence Spectrometer, including operating conditions and classifications.

Warning Labels on the Instrument

Explains the meaning of caution and high voltage labels found on the instrument.

Further assistance

Provides contact information for technical assistance.

Introduction

Specifications of the LS 55

Details the technical specifications of the LS 55 Luminescence Spectrometer, including principle, source, detectors, and monochromators.

Definitions

Defines key terms used in the manual, such as OPERATOR and RESPONSIBLE BODY.

Unpacking and Installation

Shipping kit list

Lists the part numbers and descriptions of items included in the shipping kit.

Removal of the shipping clamps

Provides step-by-step instructions for removing shipping clamps before operating the instrument.

Electrical Connections

Details how to connect the instrument to the electrical mains supply and accessory connectors.

Rear panel connectors

Describes the RS232C controller socket and terminal block on the rear panel.

Switching on the LS 55

Explains the procedure for safely switching the LS 55 instrument and the PC on.

Accessories: Installation and Use

Single Position Cellholder (standard)

Describes the standard cellholder, its specifications, and installation/maintenance.

Stirred Single Position Cellholder

Details the stirred cellholder, its description, installation, maintenance, and operation.

Biokinetics Accessory

Explains the Biokinetics accessory, its features like temperature sensing and event marking.

Plate Reader

Covers the installation and operation of the Plate Reader accessory for microplates and flat samples.

The Fast Filter Accessory

Introduces the Fast Filter accessory for cell biology studies and its spectral properties.

The Polarizer Accessory

Describes the polarizer accessory for polarization and anisotropy measurements.

The Low Temperature Accessory

Details the Low Temperature Accessory for measurements at liquid nitrogen temperature (77 K).

RS232 Commands

Section 1- RS232C Communications

Explains the RS232C interface, communication parameters, and command syntax.

Section 2 - Command Formats

Lists and describes various error codes and command formats used with the instrument.

System Description and Maintenance

The Optical System

Describes the optical path, excitation and emission monochromators, and spectral ranges.

The Electronics System and Signal Handling

Illustrates the electronics diagram and signal path from photomultipliers to the PC.

Instrument Modes

Explains the operating modes: Fluorescence, Phosphorescence, and Chemi- and Bio-luminescence.

Removing the Main Cover

Details the procedure for safely removing the instrument's main cover.

Changing the Lamp

Provides instructions for replacing the instrument's lamp.

Changing the Instrument Operating Voltage

Explains how to change the instrument's operating voltage by altering transformer connections.

Changing the mains fuse

Details how to change the mains fuse according to the operating voltage.

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