4
Tip Immersion Depth
The recommended depth for tip insertion into the sample for
each Pipet-Lite volume is shown below.
Pipet-Lite Volume Range Immersion Depth
2 µL 0.1 - 2 µL 1-2 mm
10 µL 0.5 - 10 µL 1-2 mm
20 µL 2 - 20 µL 2 - 3 mm
100 µL 10 - 100 µL 2 - 3 mm
200 µL 20 - 200 µL 2 - 4 mm
1000 µL 100 - 1000 µL 2 - 4 mm
2000 µL 200 - 2000 µL 2 - 6 mm
5000 µL 500 - 5000 µL 6 - 10 mm
10 mL 1 mL - 10 mL 6 - 10 mm
20 mL 2 mL - 20 mL 6 - 10 mm
Tip immersion depth is critical. If these depths are exceeded,
the volume measured may be inaccurate, possibly out of
specification. Tip angle is also important. Hold the pipette
within 20 degrees of vertical.
Operation
Before pipetting valuable samples, it is a good idea to prac-
tice aspirating and dispensing water before pipetting with
actual samples.
1. Set the desired volume as described on page 2.
2. Attach a new RAININ tip. Press the shaft into the tip with
only sufficient force to make a good seal.
3. Press the plunger button to the
FIRST STOP, and hold it in
this position. The magnetic latch will help you sense and
hold this position.
4. Holding Pipet-Lite vertically, place the tip into the sample
to the proper depth and relax your thumb pressure on the
plunger. The light piston spring will move the piston
upward, aspirating sample. Do not let go of the plunger
button, or the piston may snap up quickly, resulting in
inaccurate measurement.
5. Pause briefly (longer for macrovolume pipettes) to ensure
that the full volume of sample is drawn into the tip.
6. Withdraw the tip from the sample. If any liquid remains on
the outside of the tip, wipe it carefully with a lint-free tis-
sue, taking care not to touch the tip orifice.
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