Dispensing:
1. Touch the tip end against the side wall of the receiving
vessel and press the plunger slowly, past the
FIRST STOP,
to blowout (bottom of stroke.) Wait: 1 second for
2–200 µL volumes, 1-2 seconds for 1000 µL and larger.
(Longer for viscous solutions.)
2. Still holding the plunger, withdraw the tip, sliding it along
the wall of the vessel. Release the plunger.
3. Press the tip ejector button lightly to discard the tip. Use a
new tip for each sample to prevent carry-over. Repeat for
the next pipetting cycle.
Pipetting Guidelines
Pipet-Lite pipettes incorporate several new features which
enhance pipetting consistency. You should also maintain:
1. Consistent pickup and dispense rhythm.
2. Consistent speed and smoothness when pipetting.
3. Consistent pressure on the plunger button at the
FIRST
STOP
.
4. Consistent immersion depth. See table on page 4.
5. Pipette vertically, or within 20° of vertical.
6. Don’t invert or lay the pipette flat with liquid in
the tip.
Pre-Rinsing Recommended
Some solutions may leave a film on the inside tip wall. This
film remains relatively constant in successive pipettings with
the same tip, so excellent precision can be obtained by re-
filling the tip and using the refilled volume as the sample.
Successive samples from this same tip will exhibit good
reproducibility.
Reverse Mode Pipetting
Another way of reducing error due to film retention, especially
useful for more viscous liquids, is reverse mode pipetting. The
operating sequence is reversed:
1. Mount a disposable tip on the pipette shaft.
2. Press the pushbutton fully to the
SECOND STOP.
3. Immerse the tip in liquid and allow the button to return
slowly to the fully
UP position. Wait a moment for the liq-
uid column to reach equilibrium in the tip.
5
To Next Page
Loading...