6
4. Wipe any excess liquid from the outside of the tip without
touching the orifice.
5. To dispense, rest the end of the tip against the vessel wall
and press the plunger to the first stop. Hold this position a
few seconds, or long enough for the liquid column to
reach equilibrium again.
6. Remove the tip from the receiving vessel without blowing
out the remaining liquid.
7. Return excess sample in the tip to the original sample
container, if desired. Discard the used tip.
Pipetting Liquids of Varying Density
Pipet-Lite lets you compensate for solutions of density much
different from water, by setting the volume slightly higher or
lower than that required. The compensation amount must be
determined empirically.
For example, if pipetting 10 µL of CsCl solution, you deter-
mine that the volume delivered is actually 8.5 µL (≥5 sam-
ples). Change the volume setting to 11.8 µL and repeat the
measurements. If the volumes delivered are still not close
enough to 10 µL, make another slight volume adjustment
until the measurements are as desired.
Temperature Considerations
Warm or cold liquids can be measured with good precision
by using a consistent pipetting rhythm. This will help mini-
mize any differences in heating or cooling effects within the
pipette.
Use a new tip each time for best accuracy and precision
when measuring samples with temperatures greatly different
from ambient, and do not pre-rinse. As with any air-displace-
ment pipette, you will get best results if there is no delay
between picking up the sample and dispensing it.
Autoclaving
Autoclavable parts of Pipet-Lite are the shaft and the tip ejector:
121°C, 1 bar, 15-20 minutes.
Do not autoclave the complete pipette or any parts
other than the shaft and the tip ejector.
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