REPLIGEN OPUS 14 - Pressure Increase During Run; Pressure Drop During Run

To Next Page

To Previous Page

UG-OPUS® 5-80R

OPUS® 5-80R Pre-packed Chromatography Columns

• Reduce the flow rate of solution through the column to abide by pressure limit of the

packed resin bed

• Confirm that high viscosity solutions are not being used during pressure evaluation

• Flow of alcohols through the column is known to increase column pressure

• Allow all buffers and the column to equilibrate to ambient temperatures

5.3 Pressure increase during run

Causes

• Product or precipitates clogging of the polypropylene mesh

• Operation under higher flow rate than recommended for the packed resin

• Residue build up at the top of the column

• Use of high viscosity solutions or high product load concentrations

• Fouled chromatography resin

• Temperature shifts between buffers used in the column

Potential Fixes

• Clean the column with the appropriate cleaning method for the residue that clogged the

mesh and/or resin. Running in reverse flow, or up-flow mode is recommended

• Flow >5CV of equilibration buffer through the column in reverse flow. Recheck pressure and

column performance (Efficiency, Asymmetry, Pressure vs. Flow) under normal operating

conditions for comparison to results for on CoA

• Check valve position

• Allow all buffers and the column to equilibrate to ambient temperatures

5.4 Pressure drop during run

Causes

• Line or fitting leaks

• Temperature shifts during buffer transitions

• Viscosity shifts during buffer transitions

Potential Fixes

• Check lines and connections

• Allow all buffers and the column to equilibrate to ambient temperatures