UG-OPUS® 5-80R
OPUS® 5-80R Pre-packed Chromatography Columns
Appendices 6
6.1 Appendix 1: Use of a 3-Way Valve for Inlet Connection and Air Purging
To purge air when the column is first connected to the chromatography system:
1. Connect one end of the 3-way valve to the column inlet and the other end to the
chromatography system pump. Leave the column outlet closed.
2. Configure the 3-way valve flow path as shown in Figure 6 below.
Figure 6. Position of 3-way valve for purging air from the column inlet
3. OPUS® 5 – 14 Columns, attach a syringe to the purge line while pumping the mobile phase at
low flow rate (i.e. 50 cm/hr), and draw the plunger to create negative pressure. Air bubbles
will be drawn into the syringe, and mobile phase will immediately fill the space created.
4. OPUS® 20 – 80R Columns, begin pumping mobile phase at a slow flow rate (i.e. 50 cm/hr).
The mobile phase will travel into the column inlet and out of the purge line. This path will
engage and dislodge any air bubbles trapped in the column inlet line.
5. After all the air has been purged from the inlet line, engage the 3-way valve as shown in
Figure 7 below.
Figure 7. 3-way valve for flow to column
6. With the flow off and the 3-way valve configured as shown in Figure 7, open the column
outlet and connect it to the chromatography system.
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