UG-OPUS® 5-80R
OPUS® 5-80R Pre-packed Chromatography Columns
Efficiency Definitions:
Purpose: This section describes the conversion of HETP values expressed in centimeters to Efficiency
values expressed in Plates/meter. Both HETP and Efficiency may be used to quantitatively describe
the quality of a chromatography column’s packed bed.
N: Number of theoretical plates
HETP: Height of an equivalent theoretical plate, expressed in units of cm/N. Smaller values indicate
a sharper peak.
Plates/meter (N/m): Number of theoretical plates per meter. Larger values indicate a sharper peak.
Formula: N/m = 100 / HETP
Example:
Given an HETP specification of ≤0.05 cm/N, the conversion follows as below.
1
0.05
= 20
20
∗
100
1
= 2000
Since the formula takes the reciprocal of ≤0.05 cm/N, the inequality inverts to greater than or equal
to (≥). Therefore, the converted Efficiency specification is ≥2000 N/m.
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